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New Forum Archives (2009-): :
931. metylated cloning vector - restriction digest of the insert (reply: 2)
933. invitro mutagenesis using stragene kit - (reply: 4)
935. SM10 lambda pir covers my plates - U$ 10.00 Amazon.com gift card for the most helpful feedback (reply: 4)
937. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
939. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
941. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
943. Chemical competent E.coli always from single colony ? - (reply: 2)
945. Strange results of cloning - insert always reverse-oriented (reply: 2)
947. transfection or infection - difference? (reply: 1)
949. No colonies after transformation! - (reply: 2)
951. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
953. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
955. Digestion of mini-prep by mistake - (reply: 3)
957. Cohesive end filling after CIAP treatment - (reply: 2)
959. questions about transformation - (reply: 4)
933. invitro mutagenesis using stragene kit - (reply: 4)
935. SM10 lambda pir covers my plates - U$ 10.00 Amazon.com gift card for the most helpful feedback (reply: 4)
937. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
939. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
941. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
943. Chemical competent E.coli always from single colony ? - (reply: 2)
945. Strange results of cloning - insert always reverse-oriented (reply: 2)
947. transfection or infection - difference? (reply: 1)
949. No colonies after transformation! - (reply: 2)
951. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
953. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
955. Digestion of mini-prep by mistake - (reply: 3)
957. Cohesive end filling after CIAP treatment - (reply: 2)
959. questions about transformation - (reply: 4)