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New Forum Archives (2009-): :
691. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
693. Plasmid digestion after transformation - (reply: 4)
695. PCRed on restriction sites -- how do I purify it? - (reply: 8)
697. Smear above plasmid dna - (reply: 3)
699. Bacterial Induction - (reply: 1)
701. smear restriction digest - (reply: 1)
703. No DNA after midiprep - (reply: 2)
705. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
707. Impact of primer sequence on TA cloning - (reply: 3)
709. overlapping PCR protocol - (reply: 12)
711. Cloning two genes in the same plasmid - (reply: 4)
713. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
715. shRNA to primary keratinocytes - (reply: 1)
717. Ligation gel picture interpretation - (reply: 4)
719. Cloned sequence is not predicted size by western blot - (reply: 1)
693. Plasmid digestion after transformation - (reply: 4)
695. PCRed on restriction sites -- how do I purify it? - (reply: 8)
697. Smear above plasmid dna - (reply: 3)
699. Bacterial Induction - (reply: 1)
701. smear restriction digest - (reply: 1)
703. No DNA after midiprep - (reply: 2)
705. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
707. Impact of primer sequence on TA cloning - (reply: 3)
709. overlapping PCR protocol - (reply: 12)
711. Cloning two genes in the same plasmid - (reply: 4)
713. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
715. shRNA to primary keratinocytes - (reply: 1)
717. Ligation gel picture interpretation - (reply: 4)
719. Cloned sequence is not predicted size by western blot - (reply: 1)