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New Forum Archives (2009-): :
601. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
603. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
605. oligo continually appears less on gel compared to other same load - (reply: 2)
607. plasmid tetR and tet promoter - (reply: 2)
609. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
611. Digest for insert orientation - (reply: 4)
613. Problem with ligating a small insert (LoxP site) - (reply: 5)
615. molecular cloning - (reply: 3)
617. Sub-cloning and transformation - (reply: 17)
619. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
621. problems with midiprep - (reply: 3)
623. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
625. fusion of cDNA expressing protein with gfp - (reply: 1)
627. Problem with plasmids transformation - (reply: 6)
629. problem of restriction enzyme digestion - (reply: 3)
603. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
605. oligo continually appears less on gel compared to other same load - (reply: 2)
607. plasmid tetR and tet promoter - (reply: 2)
609. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
611. Digest for insert orientation - (reply: 4)
613. Problem with ligating a small insert (LoxP site) - (reply: 5)
615. molecular cloning - (reply: 3)
617. Sub-cloning and transformation - (reply: 17)
619. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
621. problems with midiprep - (reply: 3)
623. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
625. fusion of cDNA expressing protein with gfp - (reply: 1)
627. Problem with plasmids transformation - (reply: 6)
629. problem of restriction enzyme digestion - (reply: 3)