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New Forum Archives (2009-): :
31. propagating commercial chemically competent cells - (reply: 2)
33. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
35. Can someone please explain this to me? - (reply: 2)
37. TetA as a counter-selection marker - (reply: 1)
39. pET32b+ vector issue - (reply: 1)
41. Splicing DNA fragments - (reply: 2)
43. Cloning issue with bacteria growing in primary media but not in secondary - (reply: 3)
45. Luciferase assay cloning - (reply: 2)
47. After Gibson assembly or T4-Ligation obtained colonies do not grow anymore - (reply: 1)
49. Problems with ligation - (reply: 2)
51. SLIC Cloning Failure - (reply: 2)
53. how to check for intron-exon junctions - (reply: 2)
55. Cleaning loading dye for PCR sequencing - (reply: 1)
57. Bacterial knockout by double crossover - (reply: 4)
59. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
33. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
35. Can someone please explain this to me? - (reply: 2)
37. TetA as a counter-selection marker - (reply: 1)
39. pET32b+ vector issue - (reply: 1)
41. Splicing DNA fragments - (reply: 2)
43. Cloning issue with bacteria growing in primary media but not in secondary - (reply: 3)
45. Luciferase assay cloning - (reply: 2)
47. After Gibson assembly or T4-Ligation obtained colonies do not grow anymore - (reply: 1)
49. Problems with ligation - (reply: 2)
51. SLIC Cloning Failure - (reply: 2)
53. how to check for intron-exon junctions - (reply: 2)
55. Cleaning loading dye for PCR sequencing - (reply: 1)
57. Bacterial knockout by double crossover - (reply: 4)
59. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)