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New Forum Archives (2009-): :
841. how to add 3XHA to the N-terminal of a protein - (reply: 5)
843. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
845. dephosphorylate sticky-ended vector - is it necessary? (reply: 2)
847. Questions about pIRES-hrGFP1a - (reply: 1)
849. Cloning into lentiviral vector - (reply: 11)
851. use of pGEM -tT easy vectors - (reply: 2)
853. ligating a 6 His tag to plasmid - (reply: 2)
855. ligation and transformation problem - (reply: 1)
857. Mini prep of frozen bacteria cells - (reply: 2)
859. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
861. PCR clonning - (reply: 3)
863. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
865. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
867. Transformation & Minimal Plates - (reply: 5)
869. Subcloning Problem - (reply: 3)
843. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
845. dephosphorylate sticky-ended vector - is it necessary? (reply: 2)
847. Questions about pIRES-hrGFP1a - (reply: 1)
849. Cloning into lentiviral vector - (reply: 11)
851. use of pGEM -tT easy vectors - (reply: 2)
853. ligating a 6 His tag to plasmid - (reply: 2)
855. ligation and transformation problem - (reply: 1)
857. Mini prep of frozen bacteria cells - (reply: 2)
859. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
861. PCR clonning - (reply: 3)
863. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
865. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
867. Transformation & Minimal Plates - (reply: 5)
869. Subcloning Problem - (reply: 3)