1651. Help with cloning a plasmid that contains repeats! - (reply: 2)
1653. Problem with the transformation - I could find the insert in plasmid when I did Gel electrophoresis (reply: 1)
1655. Viral Shuttle Plasmid Restriction digests - (reply: 1)
1657. need help with klenow - (reply: 4)
1659. How to control cloned cDNA expression level in cells? - (reply: 8)
1661. Equation for calculating insert:vector ratio in ligation reaction - (reply: 5)
1663. Gel purification of vector DNA - low yield - (reply: 26)
1665. Identifying cDNA insert - Basic help needed (reply: 2)
1667. Sequential restriction digestion of plasmid - (reply: 58)
1669. Troublshooting failed transformation - pLEASE hELP (reply: 19)
1671. Cloning trouble - (reply: 5)
1673. Has anyone had experience with GM2163 (dam-) cells? - the enzyme BclI is blocked by dam (reply: 1)
1675. S-O-S Ligation Not Working - No Colonies After Ligation (reply: 5)
1677. What is the biggest insert for a viral vector? - (reply: 15)
1679. After Double Digestion, there is one band on the very top. What is it? - (reply: 2)

© 1999-2013 Protocol Online, All rights reserved.