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how to predict pre-miRNA and target - (Dec/18/2008 )

hi, i am a freshman in miRNA research. I would like to ask some questions.

I get some new miRNA sequences by clonging, if there is no whole genome sequence of the species, how to predict their pre-miRNA sequences and targets.

Thanks a lot!



I have found a publication that describes how to predict pre-miRNA based on miRNA sequence:
„Structural Features of miRNAs and Their Precursors” by Krol et al. published in DNA Press book.



Thank you, alchemic! Could you please introduce simply the method of predict pre-miRNA based on miRNA sequence, because i cant't find this book now.