Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Mutagenesis Related Discussions
  1. Mutagenesis PCR - (reply: 9)
  2. Mutation screening - (reply: 1)
  3. What is the current best site-direct mutagenesis Kits? - (reply: 1)
  4. Mutation in 3'UTR - (reply: 2)
  5. site directed mutagenesis - (reply: 3)
  6. Problem with Site-directed mutagenesis of a 9 kb template with high GC content ( - (reply: 3)
  7. Site-directed mutagenesis: maximum substitution - (reply: 1)
  8. Constructing deletion mutant, problems confirming - (reply: 2)
  9. Site-directed mutagenesis failed - (reply: 20)
  10. Detecting Mutation - (reply: 2)
  11. Repeated mutagensis primer in site-directed mutagenesis - (reply: 8)
  12. Frameshift mutation in DNA sequencing? - (reply: 1)
  13. what is meant by "one mutant line but from 3 different plats from a heterozy - (reply: 1)
  14. Trying to create deletion mutant. Stuck on last step. - (reply: 1)
  15. Site directed mutagenesis - (reply: 5)
  16. site-directed mutagenesis primer Tm - (reply: 4)
  17. quick change mutagenesis...... - (reply: 15)
  18. Query regarding primers for quick change mutagenesis - (reply: 3)
  19. Mutation in cloning - (reply: 6)
  20. rescue cloning of a transposon mutant - (reply: 2)
  21. quick change mutagenesis...... - (reply: 1)
  22. Question regarding yeast strain with ura3-52 mutation - (reply: 1)
  23. site-directed mutagenesis - (reply: 20)
  24. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
  25. Unexpected base after deletion mutagenesis - (reply: 3)
  26. Point mutagenesis..... - (reply: 6)
  27. Was told Mutant colonies may arise from Plates that are incubated too long - (reply: 4)
  28. aro mutant - (reply: 2)
  29. Better option, mutagenesis or gene synthesis? - (reply: 4)
  30. Repeat of desired mutation in cloning product - (reply: 1)
  31. ura3 mutant yeast strain grows on SD-uracil- - (reply: 1)
  32. Analysis of mutation - (reply: 1)
  33. genetic mutation abbreviations - (reply: 4)
  34. mutation annotations - (reply: 1)
  35. How to introduce a mutagenesis in plant? - (reply: 4)
  36. Deletion Mutant - Evolution - (reply: 16)
  37. problem with pcr mutagenesis - (reply: 10)
  38. Quick change mutagenesis No PCR product visible - (reply: 2)
  39. HPRT mutation & 6-TG selection assay - (reply: 8)
  40. Best way to genotpy a mutant gene with a 1bp difference. - (reply: 3)
  41. Is PCR efficiency higher if mutation is in the middle of primer? - (reply: 7)
  42. Site-directed mutagenesis - (reply: 14)
  43. NZY+Broth can be replaced by LB Broth in mutagenesis exp - (reply: 2)
  44. Mutation Rates - (reply: 5)
  45. No PCR product in site-directed mutagenesis - (reply: 4)
  46. question related to mutagenesis - (reply: 1)
  47. Any competent or certain E Coli for mutagenesis transformation? - (reply: 1)
  48. saturation mutagenesis library problem again - (reply: 2)
  49. shRNA rescue with site directed mutagenesis - oligo design - (reply: 4)
  50. Problems for saturation mutagenesis library - (reply: 12)
  51. Site-Directed Mutagenesis Kit; any modified protocols for plasmids larger than 1 - (reply: 3)
  52. ds-oligos mutation - (reply: 1)
  53. SLIM mutagenesis of large plasmids - (reply: 8)
  54. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
  55. Mutagenesis, deletion - what is the best kit/protocol (reply: 6)
  56. Targeted mutagenesis using a linear fragment - (reply: 1)
  57. designing mutant 3'UTR - (reply: 2)
  58. Problems with Site-Directed Mutagenesis - (reply: 2)
  59. Problem with construction of deletion mutant (Bacteria) - Deletion mutant, pK19mobsacB, Corynebacterium glutamicum (reply: 4)
  60. quantitate wild type and mutant mRNA - (reply: 1)
  61. Weird problem in in vitro mutagenesis - (reply: 1)
  62. Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit - (reply: 2)
  63. invitro mutagenesis using stragene kit - (reply: 4)
  64. invitro mutagenesis - (reply: 5)
  65. mutation using overlapping PCR, what's the success rate? - (reply: 3)
  66. PCR for MULTIPLE mutagenesis - (reply: 4)
  67. Mutagenesis PCR problem - Help with PCR mutagenesis (reply: 5)
  68. construction of a bacterial isogenic mutant - construction of a bacterial isogenic mutant (reply: 2)
  69. make a Mega plasmid mutation - (reply: 1)
  70. Site Directed Mutagenesis - Stratagene's Quickchange II XL Protocol - Oligo Issues - Melting Temperature (reply: 1)
  71. help! quikchange mutagenesis kit - (reply: 1)
  72. Non sense mutation - (reply: 1)
  73. Quickchange mutagenesis primer - (reply: 3)
  74. Mutagenesis not working - (reply: 5)
  75. Dpn1 mutagenesis - (reply: 6)
  76. PCR mutagenesis of plasmid - (reply: 5)
  77. Mutagenesis again - (reply: 1)
  78. Mutagenesis - (reply: 9)
  79. mutagenesis - start to finish - (reply: 2)
  80. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
  81. Mutagenesis troubles - (reply: 2)
  82. homozygous mutant and heterozygous mutant - (reply: 2)
  83. PCR: Smear bands, no amplification, got it all.. - PCR Mutagenesis (reply: 5)
  84. the necessity of microRNA mutation design - (reply: 1)
  85. Mutagenesis woes - (reply: 3)
  86. conditional mutagenesis - (reply: 1)
  87. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
  88. point mutation when transformed in BL21 - (reply: 2)
  89. weird site directed mutagenesis products - (reply: 3)
  90. mutagenesis by PCR or just use a kit - (reply: 5)
  91. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
  92. Insertion Mutation in Insert Sequence - (reply: 2)
  93. single amino acid mutation effects on protein function - (reply: 1)
  94. Site-directed mutagenesis (repeat oligo insert) - Strange mutagenesis reults! (reply: 10)
  95. problem with site directed mutagenesis - (reply: 4)
  96. Problems with Quick change lightning site directed mutagenesis kit(STRATAGENE) - (reply: 2)
  97. Best method for Mutagenesis? - multisite mutagenesis (reply: 2)
  98. Help complementing my mutant in trans - help me find a plasmid (reply: 1)
  99. Site directed mutagenesis - Site directed mutagenesis (reply: 1)
  100. Analyse mutant clone - (reply: 7)
  101. mutagenesis expert come in~ - (reply: 3)
  102. mutagenesis expert come in~ - (reply: 11)
  103. Problem with site directed mutagenesis - (reply: 7)
  104. Problems with Quick change lightning multi site directed mutagenesis kit - (reply: 10)
  105. Question about site directed mutagenesis - (reply: 1)
  106. Site directed mutagenesis - (reply: 2)
  107. Mutation during transformation/maxiprep - (reply: 1)
  108. Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
  109. Mutagenesis PCR and undesired amplification - (reply: 2)
  110. mutagenesis - (reply: 1)
  111. Preparing mutated MDCK cells - method of mutation of MDCK cells (reply: 1)
  112. Quikchange Mutagenesis Help Please!!! - (reply: 7)
  113. random cleavage of plasmids - insertional mutagenesis (reply: 2)
  114. Why is km important in mutant enzymes? - (reply: 3)
  115. Problems with Stratagene Site Directed Mutagenesis - (reply: 36)
  116. bacterial strains - L.lactis MG1363(^Htra mutant) or L.lactis NZ9000(^Htra mutant) (reply: 1)
  117. how to design PCR site-directed multiple mutagenesis - (reply: 1)
  118. mutation study - DNA or mRNA sequence (reply: 1)
  119. mutation in secreted protein - (reply: 5)
  120. Mutation - Δ/Δ (reply: 4)
  121. Beta-thalassemia treated in mice with antisense - Morpholino oligos block mutant splice site, restore wild-type splicing (reply: 15)
  122. Difference between SNP and point mutation (Archived) - (reply: 1)