Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Competent cells Related Discussions
  1. propagating commercial chemically competent cells - (reply: 2)
  2. Does over-expression protein under T7 promoter in Top10 work? - (reply: 1)
  3. Alternative competent cells for QuikChange kit - (reply: 2)
  4. Differences between DH5a strain of E. Coli and BL21 strain of E.Coli - (reply: 2)
  5. TOP10F´ Chemically Competent E. coli - (reply: 1)
  6. Competent cells with Hanahan's method - (reply: 2)
  7. Top10 and DH10B need IPTG for induction? - (reply: 1)
  8. Checking for efficieny competent cells - (reply: 1)
  9. freezing competent cells without dry ice or liquid N - (reply: 3)
  10. Expansion of commercial competent bacterial cell lines? - (reply: 2)
  11. What is your favorite method for making chemically competent E.coli cells? - (reply: 7)
  12. DH5alpha transformation protocol - (reply: 1)
  13. Selection of E.coli competent cells - (reply: 2)
  14. competent cells - (reply: 1)
  15. How to select competent E. coli cells? - (reply: 7)
  16. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
  17. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
  18. my competent cell grow on any agar plate - (reply: 7)
  19. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
  20. Competent bacterial cells - (reply: 2)
  21. competent cells: CaCl2 autoclave vs filter sterilisation - (reply: 12)
  22. SURE E. coli competent cells - (reply: 1)
  23. Clumpy competent cells - (reply: 3)
  24. competent cell formation - (reply: 1)
  25. An improve method for making Competent Cells without heat shock. How? - (reply: 2)
  26. Using normal competent cells in electroporation method? - (reply: 1)
  27. recover old transformed DH5 - (reply: 1)
  28. Competent cells question: JM101 vs DH5alpha - (reply: 2)
  29. Recommended competent cell for transformation of large plasmids? - (reply: 4)
  30. Making BL21 competent cells - (reply: 2)
  31. protocol for making e.coli competent cells under low temperature - (reply: 1)
  32. Any competent or certain E Coli for mutagenesis transformation? - (reply: 1)
  33. competent cells - (reply: 2)
  34. SURE or Stbl competent cells for retroviral vector? - (reply: 2)
  35. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
  36. picking up colonies and growing in the broth after transformaion - cloning with competent cells (reply: 1)
  37. competent cells stock - (reply: 3)
  38. DH5alpha cells - (reply: 4)
  39. growing competent cells - (reply: 1)
  40. making more competent cells - (reply: 2)
  41. Agrobacterium EHA101 competent cells - (reply: 1)
  42. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
  43. One Shot TOP10 Cells - (reply: 6)
  44. DH5 Alpha Lambda pir and Conjugation - (reply: 1)
  45. Chemical competent E.coli always from single colony ? - (reply: 2)
  46. Freezing home-brewed competent cells - Dry-ice first or straight to -80 C freezer? (reply: 6)
  47. DH5 Alpha Transformation - (reply: 2)
  48. tranformation trouble using dh5alpha - (reply: 1)
  49. Starting cells for making one's own competent cells - (reply: 7)
  50. what is the difference between DH10B and Top10 - (reply: 1)
  51. Competent cell storage for long term use - (reply: 2)
  52. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
  53. Tips on fermenting DH5alpha and MG1655 - e.coli cells never leave lag phase during fermentation (reply: 1)
  54. competent cells - OD600 reading (reply: 6)
  55. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
  56. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
  57. BL21(DE3)LysS competent cells - (reply: 1)
  58. CaCl2 for making competent cells - (reply: 3)
  59. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
  60. dh5 alpha competent cells? - (reply: 2)
  61. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
  62. pre-prepararation of competent cells - (reply: 3)
  63. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
  64. z competent cells - (reply: 2)
  65. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
  66. T7 and DH5 alpha - Help me clear out some things (reply: 3)
  67. Making competent cell from freeze-thawed stock - (reply: 2)
  68. PCR problems on high GC content gene - Trouble with Colony PCR of TOP10 transformants w/ TOPO-vector (reply: 4)
  69. Starter culture dilution (preparation of competent cells) - I don't know how much should I dilute my starter culture... (reply: 3)
  70. Chosing the right competent cells - (reply: 1)
  71. Picking strain for e coli competent cells - (reply: 6)
  72. competent cell preparation? - (reply: 1)
  73. One Shot® ccdB Survival™-T1R Chemically Competent Cells: Gateway cloning???? - problems using competent cells for destination vectors in gateway clon (reply: 5)
  74. Competent Cells - (reply: 5)
  75. Plyse cell and DH5a - (reply: 1)
  76. COMPETENT CELL PREPARATION - (reply: 6)
  77. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
  78. where can I find lamda-pir DH5a? - (reply: 3)
  79. Storage of invitrogen top10 cells - (reply: 11)
  80. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
  81. Growing of TOP10 chemically competent cells - (reply: 4)
  82. What does the DH5alpha mean in E.coli DH5alpha bacteria - (reply: 10)
  83. Competent DH5 alpha - (reply: 4)
  84. DH5a comp cells - (reply: 5)
  85. Chemically competent to electrocompetent DH5alpha - (reply: 1)
  86. To what extent competent cells be thawed before transformation? - (reply: 4)
  87. contamination in transformed bacteria - black filamentous fungi grows when culturing competent bacteria with m (reply: 8)
  88. making competent E coli DH5a cells - (reply: 6)