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New Forum Archives (2009-)
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ELISA-and-Immunoassay
1.
Accidentally left some serum out overnight at room temp.... -
(reply: 3)
2.
Establish of an indirect ELISA in one month possible -
(reply: 2)
3.
Is there a maximum blocking time? -
(reply: 2)
4.
Re use the pre coated microtiter plate 96 wells -
(reply: 2)
5.
Sample storage for ELISA (cell culture supernatant) -
(reply: 2)
6.
Help needed! ELISA reproducibility issues? -
(reply: 2)
7.
ELISA, BDNF, Biotinylated Conjugate -
(reply: 10)
8.
Biotinylated BDNF -
(reply: 2)
9.
What is the difference between measurment range and indication range in ELISA re -
(reply: 1)
10.
ELISA normalization for relative quantification -
(reply: 6)
11.
Human IgG Elisa variability problems -
(reply: 2)
12.
Green-dye in HRP-Conjugate solution -
(reply: 7)
13.
Does curve height/range of absorbance values matter? -
(reply: 2)
14.
ELISA SETUP,negative control OD is higher than tests -
(reply: 7)
15.
serum cytokine detection -
(reply: 1)
16.
Protein precipitation from RIPA buffer to perform ELISA -
(reply: 4)
17.
Double coating in Sandwich ELISA - Problems -
(reply: 2)
18.
ELISA quantification -
(reply: 2)
19.
Can I test protein denaturation after sonication -
(reply: 2)
20.
Plasma samples with lower OD than blank -
(reply: 5)
21.
ELISA - use of technical or biological replicates? -
(reply: 3)
22.
ELISA Incubator -
(reply: 3)
23.
Inflammatory cytokine ELISA-kit on homeogenates from small biopsies? -
(reply: 3)
24.
Culture Supernatant Gives O.D even in the uncoated wells -
(reply: 4)
25.
e-cadherin and calcium in an elisa -
(reply: 1)
26.
HIV p24 ELISA - problem with high background -
(reply: 4)
27.
Basic Novateinbio ELISA protocol -
(reply: 1)
28.
ELISA on DMSO treated or Formalin Fixed samples - Experiences? -
(reply: 3)
29.
protein concentration/well calculation -
(reply: 1)
30.
Seeking advice on coated plates storage -
(reply: 5)
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