Protocol Online logo
Top : New Forum Archives (2009-): : ELISA and Immunoassay

how to analyze endpoint elisa - (Mar/11/2015 )

I was wondering how do i analyze endpoint elisa.


I have two samples and want to compare their antibody response, but I don't have standard, so I did endpoint, but I don't know how to analyze the results based on the OD value. 


Can anybody help me /


Endpoint Elisa assay with quantitative analysis : calculation of unknown concentrations based on a standard curve.

                                   with qualitative  analysis:  unknowns sorted using one or multiple cut-off values.

i hope this can help you, if you wanna learn more, you can find some details in some libraries and wiki. these are very wonderful assistant ways.