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Protocols
- Methods for DNA Isolation (The Minion Lab, College of Veterinary Medicine at Iowa State University)
This page, adapted from Bruce A. Roe's protocol,contains several methods for plasmid and genomic DNA isolation:
Large scale double-stranded DNA isolation
Midiprep double-stranded DNA isolation
Miniprep double-stranded DNA isolation
Large scale M13RF isolation
Single-stranded M13 DNA isolation using phenol
Genomic DNA isolation from blood
http://mycoplasmas.vm.iastate.edu/lab_site/methods...
Added: Tue Nov 16 2004, Hits: 9516, Reviews: 0 Write review Cached -
DNA extraction from Cheek Cells (mouth wash)
(DNA Laboratory, Medical School)
A modification of the salting out procedure for extracting DNA from cheek cells
http://geneticslab.topcities.com/cheekcells.htm
Added: Sun Mar 14 2004, Hits: 1540, Reviews: 0 Write review Cached -
Extracting Mitochondrial DNA (mtDNA) from Hair
(Molecular Ecology and Evolution Lab (MEEL), James Cook University, Australia)
This method can be used to extract mtDNA from hair shafts; if the follicle is present it should also extract nuclear DNA.
http://www.protocol-online.org/files/protocol_4335...
Added: Tue Feb 13 2007, Hits: 2356, Reviews: 0 Write review
Trouble Shootings and FAQs
- how to purify small fragment of DNA?
- How to isolate single-stranded DNA from mammalian?
- Tips for breaking up tissue in liquid nitrogen for nucleic acid extraction
- Using CTAB for polysaccharide removal during DNA extraction
- Why is TRIS used in DNA extraction?
- Failed to extract DNA from urine
- how to clean DNA>10KB?