Top : Molecular Biology : DNA : DNA Extraction & Purification : DNA Extraction from Agarose Gel
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  • DNA extraction from agarose gels (Matt Lewis, Department of Pathology, University of Liverpool )
    Very nice protocol which covers three methods of extracting DNA from agarose gel: Paper strip method, Spin-columns and Dialysis tubing (semi-permeable membrane, Visking tubing)
    http://www.methodbook.net/dna/gelextrc.html
    Added: Fri Sep 06 2002, Hits: 12982, Reviews: 0  Write review   Cached
  • DNA Purification from Agarose Gels (John Roth, Division of Biological Sciences,University of California at Davis)
    DNA Purification using DEAE nitrocellulaose membranes.
    http://rothlab.ucdavis.edu/protocols/P01.html
    Added: Tue May 25 2004, Hits: 5397, Reviews: 0  Write review   Cached
  • Recovering DNA from Agarose Gels (John Roth, Division of Biological Sciences,University of California at Davis)
    A review on various methods of recovering DNA from agarose gels. Choice of extraction procedure is dependent on what the DNA is to be used for afterward. Typically, methods involving extraction with organic solvents, electroelution, or binding of the DNA to silica particles or ion-exchange resins give quite pure DNA, but yields are relatively low. The apparently poor yield of DNA recovered by the spin techniques has been found to be clean enough for ligating directly from the eluate without organic extraction or any other further manipulation. On the other hand, high-yield techniques tend to be problematic in enzyme reactions.
    http://rothlab.ucdavis.edu/protocols/DNA-from-Agar...
    Added: Tue May 25 2004, Hits: 6387, Reviews: 0  Write review   Cached
  • Alternate Method for Purifying DNA From Agarose Gels (Julie B. Wolf, University of Maryland, Baltimore County)
    http://www.research.umbc.edu/~jwolf/m9.htm
    Added: Tue May 14 2002, Hits: 3501, Reviews: 0  Write review   Cached
  • DNA Extraction from Agarose Gel (LaboratoryExperiments.com)
    Protocol for extracting DNA from agarose gel using fileter.
    http://www.laboratoryexperiments.com/LAB9.htm
    Added: Tue May 14 2002, Hits: 4167, Reviews: 0  Write review   Cached
  • DNA Extraction from Agarose  (The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
    Glass wool spin column method
    http://labs.fhcrc.org/hahn/Methods/mol_bio_meth/dn...
    Added: Tue May 14 2002, Hits: 2883, Reviews: 0  Write review   Cached
  • DNA Extraction from Agarose  (The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
    Glass wool spin column method
    http://labs.fhcrc.org/hahn/Methods/mol_bio_meth/dn...
    Added: Tue May 14 2002, Hits: 2070, Reviews: 0  Write review   Cached
  • DNA Fragment Purification from Agarose or Acrylamide (Mike A. Dyer)
    For fragments from 200 bp to 10 kb the agarose purification is ideal. For smaller fragments (20 bp to 400 bp) the acrylamide purification is preferred.
    http://axon.med.harvard.edu/~cepko/protocol/mike/D...
    Added: Tue May 14 2002, Hits: 2115, Reviews: 0  Write review   Cached
  • DNA Isolating From Low Melting-Temperature Agarose (NWFSC)
    Phenol-based Method for the Isolation of DNA Fragments from Low-Melting Temperature Agarose
    http://micro.nwfsc.noaa.gov/protocols/lmt.html
    Added: Tue May 14 2002, Hits: 2022, Reviews: 0  Write review  
  • DNA Isolation from Agarose Gel Using NaI (Corbett Lab)
    http://www.biochem.emory.edu/labs/acorbe2/protocol...
    Added: Tue May 14 2002, Hits: 1367, Reviews: 0  Write review  
  • DNA Isolation from Agarose Gels with DEAE Paper  (Hancock Lab)
    Detailed protocol with recipes
    http://www.cmdr.ubc.ca/bobh/methods/ISOLATIONOFDNA...
    Added: Tue May 14 2002, Hits: 778, Reviews: 0  Write review   Cached
  • DNA Purification from Gels (John Roth, Division of Biological Sciences,University of California at Davis)
    Simple freezing method for recovering DNA from gels.
    http://rothlab.ucdavis.edu/protocols/P02.html
    Added: Tue May 25 2004, Hits: 2364, Reviews: 0  Write review   Cached
  • Elution of DNA fragments from agarose (Roe, OU)
    http://www.genome.ou.edu/protocol_book/protocol_pa...
    Added: Tue May 14 2002, Hits: 1972, Reviews: 0  Write review   Cached
  • Gene-Kleen Protocol (Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
    Purification of DNA from agarose gels is an essential method involved in the sub-cloning of DNA fragments. This method describes a variation of the method of Vogelstein and Gillespie, 1979 (Proc. Natl. Acad. Sci. USA. 76, (2) 615-619) involving the use of sodium iodide.
    http://www.nottingham.ac.uk/~mbzspd/methods/Gene-K...
    Added: Sat Apr 10 2004, Hits: 150, Reviews: 0  Write review   Cached
  • Isolation of DNA from Agarose Gels (Paper Slurry Method) (Kitto Lab, The University of Texas at Austin)
    This procedure isolates DNA from agarose gels by filtration through a filter-paper column. The column is made in a 500 µL tube from a slurry of filter paper in TE buffer.
    http://kitto.cm.utexas.edu/research/Kittolabpage/P...
    Added: Tue Oct 08 2002, Hits: 1050, Reviews: 0  Write review   Cached
  • Isolation of Restriction Fragments from Agarose Gels by Collection onto DEAE Cellulose (Donis Keller Lab)
    Recovery of DNA from agarose gel
    http://wheat.pw.usda.gov/~lazo/methods/donis/rdm/r...
    Added: Tue May 14 2002, Hits: 406, Reviews: 0  Write review   Cached
  • Recovery of DNA from Agarose Gel with Silica (Hideaki Shiraishi, Kyoto University)
    An inexpensive alternative to glassmilk for DNA purification.
    http://kuchem.kyoto-u.ac.jp/seika/shiraishi/protoc...
    Added: Fri Jan 30 2004, Hits: 1428, Reviews: 0  Write review   Cached
  • Recovery of DNA from Low Melting Point (LMP) Agarose (Hideaki Shiraishi, Kyoto University)
    Simple method for extracting DNA from LWP gel.
    http://kuchem.kyoto-u.ac.jp/seika/shiraishi/protoc...
    Added: Fri Jan 30 2004, Hits: 1606, Reviews: 0  Write review   Cached