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- DNA extraction from agarose gels (Matt Lewis, Department of Pathology, University of Liverpool )
Very nice protocol which covers three methods of extracting DNA from agarose gel: Paper strip method, Spin-columns and Dialysis tubing (semi-permeable membrane, Visking tubing)
http://www.methodbook.net/dna/gelextrc.html
Added: Fri Sep 06 2002, Hits: 16514, Reviews: 0 Write review Cached - DNA Purification from Agarose Gels (John Roth, Division of Biological Sciences,University of California at Davis)
DNA Purification using DEAE nitrocellulaose membranes.
http://rothlab.ucdavis.edu/protocols/P01.html
Added: Tue May 25 2004, Hits: 7881, Reviews: 0 Write review Cached - Freeze Squeeze PCR Product Purification from Agorose Gel (Clare L Mckay)
The “Freeze squeeze” method is a cost effective and technically simple procedure to isolate and prepare PCR products for DNA sequencing.
Added: Tue Jul 25 2006, Reviews: 0 Write review - Isolation of DNA Fragments Using Glass Milk (The Minion Lab, College of Veterinary Medicine at Iowa State University)
Simple method for extracting DNA from agarose gel using NaI and glass milk.
http://mycoplasmas.vm.iastate.edu/lab_site/methods...
Added: Tue Nov 16 2004, Hits: 2838, Reviews: 0 Write review Cached - Recovering DNA from Agarose Gels (John Roth, Division of Biological Sciences,University of California at Davis)
A review on various methods of recovering DNA from agarose gels. Choice of extraction procedure is dependent on what the DNA is to be used for afterward. Typically, methods involving extraction with organic solvents, electroelution, or binding of the DNA to silica particles or ion-exchange resins give quite pure DNA, but yields are relatively low. The apparently poor yield of DNA recovered by the spin techniques has been found to be clean enough for ligating directly from the eluate without organic extraction or any other further manipulation. On the other hand, high-yield techniques tend to be problematic in enzyme reactions.
http://rothlab.ucdavis.edu/protocols/DNA-from-Agar...
Added: Tue May 25 2004, Hits: 9047, Reviews: 0 Write review Cached -
Alternate Method for Purifying DNA From Agarose Gels
(Julie B. Wolf, University of Maryland, Baltimore County)
http://userpages.umbc.edu/~jwolf/m9.htm
Added: Tue May 14 2002, Hits: 4483, Reviews: 0 Write review Cached -
DNA Extraction from Agarose Gel
(LaboratoryExperiments.com)
Protocol for extracting DNA from agarose gel using fileter.
http://www.laboratoryexperiments.com/LAB9.htm
Added: Tue May 14 2002, Hits: 5621, Reviews: 0 Write review Cached -
DNA Extraction from Agarose
(The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
Glass wool spin column method
http://www.fhcrc.org/labs/hahn/methods/mol_bio_met...
Added: Tue May 14 2002, Hits: 2486, Reviews: 0 Write review Cached -
DNA Extraction from Agarose
(The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
Glass wool spin column method
http://www.fhcrc.org/labs/hahn/methods/mol_bio_met...
Added: Tue May 14 2002, Hits: 1813, Reviews: 0 Write review Cached -
DNA Fragment Purification from Agarose or Acrylamide
(Mike A. Dyer)
For fragments from 200 bp to 10 kb the agarose purification is ideal. For smaller fragments (20 bp to 400 bp) the acrylamide purification is preferred.
http://axon.med.harvard.edu/~cepko/protocol/mike/D...
Added: Tue May 14 2002, Hits: 3164, Reviews: 0 Write review Cached