Protocols
- Genomic DNA Isolation from Blood (Bruce A. Roe Lab, University of Oklahoma)
Standard protocol for DNA isolation from blood
http://www.genome.ou.edu/protocol_book/protocol_pa...
Added: Tue May 14 2002, Hits: 53056, Reviews: 0 Write review - Rapid Extraction of High Quality DNA from Whole Blood Stored at 4ºC for Long Period (Vahid Iranpur-mobarakeh, Department of Animal Science, Faculty of Agriculture, Shahrekord University, Shahrekord, Iran)
None of the procedures yielded DNA of suitable purity for SSR and other PCR assays for DNA that was stored at 4ºC on prolonged period. We established an improved procedure for rapid isolation of DNA from sheep’s blood and other species stored at 4ºC for up to one year or more and suitable for SSR analysis and other PCR-based applications
Added: Sun May 02 2010, Reviews: 0 Write review -
DNA Preparation from Blood
(Thomas Ried's Lab, NCI)
Extract DNA from whole blood (EDTA, heparin, citrate) using phenol/chloroform method.
http://www.riedlab.nci.nih.gov/publications/2438.2...
Added: Fri Oct 18 2002, Hits: 33446, Reviews: 0 Write review -
DNA extraction from peripheral blood leucocytes
(DNA Laboratory, Medical School, Malta)
A salting out procedure for extracting DNA from white cells giving good yield and good quality DNA
http://geneticslab.topcities.com/DNAextraction.htm
Added: Sat Dec 06 2003, Hits: 12896, Reviews: 0 Write review Cached -
DNA Isolation from Human Peripheral Blood
(Genomic Medicine Biorepository, Genomic Medicine Institute, Lerner Research Institute)
http://www.lerner.ccf.org/gmi/gmb/documents/GMB002...
Added: Sat Dec 08 2012, Hits: 6450, Reviews: 0 Write review -
DNA Preparation from Lymphoblastoid Cells
(Thomas Ried's Lab, NCI)
Organic extraction of DNA from cultured cells.
http://www.riedlab.nci.nih.gov/publications/2440.2...
Added: Fri Oct 18 2002, Hits: 4901, Reviews: 0 Write review -
Extraction of DNA from Blood for PCR
(Jackson Labs)
Simple method for preparing DNA from blood for PCR without organic extraction
http://www.jax.org/imr/whole_blood.html
Added: Tue May 14 2002, Hits: 13923, Reviews: 0 Write review Cached -
Extraction of DNA from the Whole Blood by Silica Gel
(Institute of Gene Biology, RAS)
Simple and inexpensive procedure in which the pure DNA is extracted from the whole blood within 15-20 min.
http://www.ras.ru/biogen/blood.html
Added: Tue May 14 2002, Hits: 583, Reviews: 0 Write review Cached - Extraction of genomic DNA from whole blood (Laura-Lee Boodram, Department of Life Sciences, The University of the West Indies)
The protocol is simple and fairly rapid. It does not require the use of organic solvents but rather utilizes salt extraction to precipitate contaminating proteins. High quality DNA is obtained suitable for immediate PCR applications. One can obtain approximately 100-200 ug of DNA from 4-8 mL of fresh or frozen whole blood.
Added: Mon May 17 2004, Reviews: 0 Write review -
Manual Isolation of Human DNA from Lymphoblasts or Whole Blood
(Donis Keller Lab)
Nucleated blood cells are used to prepare genomic DNA. The cells are first washed, then lysed and the nuclei (left intact) are pelleted in a low speed centrifuge run.
http://hg.wustl.edu/hdk_lab_manual/dna/dna1.html
Added: Tue May 14 2002, Hits: 6006, Reviews: 0 Write review Cached