Protocols
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DNA Extraction from Blood
(The Genomic Variation Laboratory, University of California in Davis)
A two stage protocol by first purifying nuclear pellets followed by extraction of DNA from nuclei.
http://genome-lab.ucdavis.edu/Protocols/dna_extrac...
Added: Mon Aug 02 2004, Hits: 26795, Reviews: 0 Write review Cached -
DNA extraction from peripheral blood leucocytes
(DNA Laboratory, Medical School, Malta)
A salting out procedure for extracting DNA from white cells giving good yield and good quality DNA
http://geneticslab.topcities.com/DNAextraction.htm
Added: Sat Dec 06 2003, Hits: 7252, Reviews: 0 Write review Cached -
DNA Preparation from Blood
(Thomas Ried's Lab, NCI)
Extract DNA from whole blood (EDTA, heparin, citrate) using phenol/chloroform method.
http://www.riedlab.nci.nih.gov/publications/2438.2...
Added: Fri Oct 18 2002, Hits: 18496, Reviews: 0 Write review -
DNA Preparation from Lymphoblastoid Cells
(Thomas Ried's Lab, NCI)
Organic extraction of DNA from cultured cells.
http://www.riedlab.nci.nih.gov/publications/2440.2...
Added: Fri Oct 18 2002, Hits: 3261, Reviews: 0 Write review -
Extraction of DNA from Blood for PCR
(Jackson Labs)
Simple method for preparing DNA from blood for PCR without organic extraction
http://www.jax.org/imr/whole_blood.html
Added: Tue May 14 2002, Hits: 15078, Reviews: 0 Write review Cached -
Extraction of DNA from the Whole Blood by Silica Gel
(Institute of Gene Biology, RAS)
Simple and inexpensive procedure in which the pure DNA is extracted from the whole blood within 15-20 min.
http://www.ras.ru/biogen/blood.html
Added: Tue May 14 2002, Hits: 2957, Reviews: 0 Write review Cached - Extraction of genomic DNA from whole blood (Laura-Lee Boodram, Department of Life Sciences, The University of the West Indies)
The protocol is simple and fairly rapid. It does not require the use of organic solvents but rather utilizes salt extraction to precipitate contaminating proteins. High quality DNA is obtained suitable for immediate PCR applications. One can obtain approximately 100-200 ug of DNA from 4-8 mL of fresh or frozen whole blood.
Added: Mon May 17 2004, Reviews: 0 Write review -
Genomic DNA Isolation from Blood
(Roe Lab)
Standard protocol for DNA isolation from blood
http://www.genome.ou.edu/protocol_book/protocol_pa...
Added: Tue May 14 2002, Hits: 13508, Reviews: 0 Write review Cached -
Manual Isolation of Human DNA from Lymphoblasts or Whole Blood
(Donis Keller Lab)
Nucleated blood cells are used to prepare genomic DNA. The cells are first washed, then lysed and the nuclei (left intact) are pelleted in a low speed centrifuge run.
http://humgen.wustl.edu/hdk_lab_manual/dna/dna1.ht...
Added: Tue May 14 2002, Hits: 5910, Reviews: 0 Write review Cached -
Rapid Genomic DNA Extraction (RGDE) from Blood
(MOHAMMAD ALI SAREMI from Kawsar Human Genetic Research Center)
Nowadays expanded windows have been opened to the world especially in the area of genetic and biology science by performance of big projects such as human genome project. In this regard, one of the primary and important steps for all is DNA extraction with high quality & quantity in minimum time from cells. By using RGDE method, genomic DNA with high quality & quantity can be acquired in the shortest time which has been presented in the world up to now.
http://www.protocol-online.org/files/protocol_4791...
Added: Wed Aug 29 2007, Hits: 1222, Reviews: 0 Write review