Subcategories

Protocols

• Extrachromosomal Elements-Plasmids (Michael Blaber)
  What's plasmid? Find answer here. It provides background information on the features of plasmid, selection marker and more...
  http://wine1.sb.fsu.edu/bch5425/lect13/lect13.htm
  Added: Tue May 14 2002, Hits: 2203, Reviews: 0  Write review   Cached
• PCR Amplification of Inserts from Bacterial Cultures (CIMMYT Applied Molecular Genetics Laboratory)
  PCR amplification of plasmid insert without plasmid preparation
  http://www.cimmyt.org/english/docs/manual/protocol...
  Added: Mon Aug 05 2002, Hits: 5829, Reviews: 0  Write review  
• Plasmid Stability Test (The Protein Expression and Purification Facility, EMBL)
  Immediately before indcution, it is advisable to test the culture to determine the fraction of cells that still carry the target plasmid. This involves plating of cells on four different plates.
  http://www.embl.de/ExternalInfo/protein_unit/draft...
  Added: Sat Sep 21 2002, Hits: 1826, Reviews: 0  Write review   Cached

Trouble Shootings and FAQs

• how to remove DNA contamination from plasmids purification?
• trouble in plasmid pcr- i cannot obtain designed band from a definied plasmid
• Plasmid extraction from E.coli culture - basic questions
• How to identify plasmid- using enzyme and PCR or other?
• Problem with yeast plasmid extraction
• Low plasmid yield with HB101 strain
• Do I need to removal of RNA from plasmid prep for sequencing?
• Environmental aeromonas contamination causes deletion problem
• Strange glycerol stocks- could not get plasmid from bacteria
• How to store plasmid DNA?
• Problem with RNase treatment of plasmid