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Top : Molecular Biology : Phage
Overview: Phage or bacteriophage is a virus that can infect bacteria, leading to replication of the virus and lysis of the host bacteria. Phage DNA can serve as a basis for cloning vectors to create phage libraries containing long pieces of foreign DNA.
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Protocols
  • Phage Titer (E.T. Wurtzel, Department of Biological Sciences, Lehman College)
    Lambda phage is a commonly used vector for transgenes. Its very high rate of infectivity makes it ideal for creating large numbers of clones. Large numbers of clones are often needed for construction of gene libraries that represent large populations of molecules. These libraries are also easy to sort through, as lambda phage clones are particularly easy to array spatially at high density. This is called plating a library. This protocol will plate phage at various densities in order to generate a titer (count) and amplify a library in order isolate DNA
    http://a32.lehman.cuny.edu/molbio_course/titer.htm
    Added: Mon Aug 26 2002, Hits: 1617, Reviews: 0  Write review   Cached
  • Bacteriophage Preparation PDF file (The Morimoto Lab, Northwestern University)
    General methods of phage preparation and purification.
    http://www.biochem.northwestern.edu/ibis/morimoto/...
    Added: Mon Mar 29 2004, Hits: 1835, Reviews: 0  Write review  
  • Phage Titering (Fankhauser Lab, University of Cincinnati Clermont College)
    Illustrated procedures
    http://biology.clc.uc.edu/fankhauser/Labs/Microbio...
    Added: Sun Jul 30 2006, Hits: 814, Reviews: 0  Write review   Cached
  • Preparation of Phage Particles (Dario Neri's labs, Institute of Pharmaceutical Sciences ETHZ)
    Preparation of phage particles from phage vectors and from phagemids.
    http://www.pharma.ethz.ch/institute_groups/biomacr...
    Added: Mon Sep 09 2002, Hits: 599, Reviews: 0  Write review   Cached
  • Protocol for Phage Lysate Preparation (Lenski Lab, Michigan State University)
    This protocol is optimized for the production of high-titer T4 lysates and can also be used for phages T2, T7, T5 and T6.
    http://myxo.css.msu.edu/ecoli/phagepharm.html
    Added: Sun Jul 30 2006, Hits: 668, Reviews: 0  Write review   Cached
  • Titering a Bacteriophage Library (Karl Clark, University of Minnesota)
    This protocol is for determining the concentration of infectious phage, as represented by the number of plaque forming units per mL (pfu/mL)
    http://www.cbs.umn.edu/~kclark/protocols/titer.htm...
    Added: Sun Jul 30 2006, Hits: 353, Reviews: 0  Write review   Cached