Protocols
- Fungal Genomic DNA Extraction (Contributed by Dr. Eric W. Boehm)
This procedure does not require phenol extraction. The DNA is pure enough for restriction digests, PCR and genomic library construction.
Added: Tue May 14 2002, Reviews: 1 Read reviews Write review -
DNA extraction from Coccidioides
(Fungal Genetics Stock Center)
A safe and convenient method of extracting DNA from such fungi in which the culture is killed by steaming, allowing removal from the containment facilities, as soon as possible.
http://www.fgsc.net/fgn42/burt.html
Added: Mon Dec 09 2002, Hits: 1136, Reviews: 0 Write review Cached -
DNA Isolation for PCR from Fusarium
(Fungal Genetics Stock Center)
A method for isolating DNA without grinding from Fusarium cultures grown on agar slants using a modification of the SDS miniprep method.
http://www.fgsc.net/fgn38/duteau.html
Added: Mon Dec 09 2002, Hits: 2291, Reviews: 0 Write review Cached -
DNA Isolation from Fungal
(Lazo Lab)
http://wheat.pw.usda.gov/~lazo/methods/lazo/dnafun...
Added: Tue May 14 2002, Hits: 2415, Reviews: 0 Write review Cached -
Fast DNA extraction from Neurospora
(Fungal Genetics Stock Center)
A simple method for growing Neurospora and for isolation of DNA that may be performed in two days from start to finish. The growth of mycelia in Petri plates eliminates the need for large numbers of flasks when growing many cultures for DNA isolation
http://www.fgsc.net/fgn/camb.html
Added: Mon Nov 18 2002, Hits: 1176, Reviews: 0 Write review Cached -
Growing Fungi for DNA Extraction
(Fungal Genetics Stock Center)
Use of reverse agar for cultivation of fungi for DNA extraction
http://www.fgsc.net/fgn41/seifert.html
Added: Mon Nov 18 2002, Hits: 2166, Reviews: 0 Write review Cached -
Quick RNA Miniprep from Neurospora(ATA)
(Fungal Genetics Stock Center)
The method involves the use of a triphenylmethane dye, aurintricarboxylic (ATA), to protect the RNA
http://www.fgsc.net/fgn37/lindgren.html
Added: Mon Dec 09 2002, Hits: 622, Reviews: 0 Write review Cached -
RNA Miniprep from Neurospora
(Fungal Genetics Stock Center)
Procedures of RNA extraction published so far follow roughly three different approaches, where phenol/chloroform, guanidinium salts, and/or LiCl is used. However, these protocols have some disadvantages. This method, combining the advantages of phenol extraction and LiCl precipitation, is for isolating high quality total RNA from N. crassa mycelia that reliably yields large quantities.
http://www.fgsc.net/fgn37/sokol.html
Added: Mon Dec 09 2002, Hits: 998, Reviews: 0 Write review Cached -
Small-scale DNA prep from Neurospora
(Fungal Genetics Stock Center)
Molecular biology experiments often require preparation of small amounts of DNA from many samples. This abbreviated DNA isolation method yields an average of 0.6 micrograms of genomic DNA that is suitable for Southern analysis or PCR
http://www.fgsc.net/fgn/irelan.html
Added: Mon Dec 09 2002, Hits: 773, Reviews: 0 Write review Cached -
Total DNA Isolation from Filamentous Fungi
(Fungal Genetics Stock Center)
Most methods of DNA preparation from fungi are time-consuming due to the need to first make protoplasts, expensive for chemicals such as cesium chloride, or suitable only for small scale preparations. This protocol describes a simple method for total DNA preparation, yielding a product of quality suitable for restriction digestion and library construction.
http://www.fgsc.net/fgn37/borges.html
Added: Mon Dec 09 2002, Hits: 3049, Reviews: 0 Write review Cached