Protocols
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DNA-Miniprep - Alkaline Lysis
(A. Untergasser)
new
A great protocol to prep. DNA from Agrobacterium, it is also great for E. coli.
http://www.untergasser.de/lab/protocols/miniprep_a...
Added: Sun Nov 16 2008, Hits: 0, Reviews: 0 Write review - A Simple Protocol for DNA Extraction from Staphylococcus Aureus (Protocol Online)
In research the protocol must be consistent, repeatable, easy and cost effective. Currently there are a number of protocols for isolation of DNA from S. aureus using lysostaphin. However, these have some limitations. The proposed protocol can be informative and useful for researchers for extraction of DNA from staphylococcal cells without addition of lysostaphin. As this chemical is quite costly and increases the cost of DNA extractions in general laboratories. The protocol described here is able to extract staphylococcal DNA in sufficient quantity and quality.
Added: Sun Oct 05 2008, Reviews: 0 Write review -
Chromosomal DNA Isolation
(Hancock Laboratory Methods, Department of Microbiology and Immunology,University of British Columbia)
For bacteria chromosomal DNA
http://www.cmdr.ubc.ca/bobh/showmethod.php?methodi...
Added: Sat Aug 31 2002, Hits: 12703, Reviews: 0 Write review Cached -
DNA Extraction from Bacteria
(Lazo Lab)
Phenol/chloroform method
http://wheat.pw.usda.gov/~lazo/methods/lazo/dnagen...
Added: Tue May 14 2002, Hits: 18944, Reviews: 0 Write review Cached -
DNA Extraction from Bacteria
(Julie B. Wolf, University of Maryland, Baltimore County)
Phenol/chloroform method
http://userpages.umbc.edu/~jwolf/m1.htm#genomic%20...
Added: Tue May 14 2002, Hits: 10998, Reviews: 0 Write review Cached -
DNA Extraction From Bacteria (Triton Method)
(NWFSC)
http://micro.nwfsc.noaa.gov/protocols/tritonprep.h...
Added: Tue May 14 2002, Hits: 5149, Reviews: 0 Write review Cached -
DNA Extraction from Gram-positive Bacteria
(NWFSC)
This procedure was originally developed for Listeria monocytogenes but has worked well with other Gram+ bacteria
http://micro.nwfsc.noaa.gov/protocols/GramPosDNA.h...
Added: Tue May 14 2002, Hits: 4161, Reviews: 0 Write review Cached -
DNA-Miniprep. - Rapid boiling
(A. Untergasser)
A robust and fast method to extract DNA from bacteria in a simple way.
http://www.untergasser.de/lab/protocols/miniprep_r...
Added: Sun Mar 30 2008, Hits: 691, Reviews: 0 Write review -
E.coli Total RNA Isolation
(E. coli Genome Project at UW-Madison)
Stablize E.coli RNA with Qiagen Bacteria RNA Protect Reagent and isolate total RNA with Epicentre MasterPure RNA Purification Kit. Extract RNA for microarray
http://www.genome.wisc.edu/resources/protocols/rna...
Added: Mon Aug 05 2002, Hits: 3613, Reviews: 0 Write review Cached -
Genomic DNA Isolation
(Jeffrey D. Newman, Lycoming College)
Phenol/chloroform method, this procedure does not work well with Gram + cocci
http://srv2.lycoming.edu/~newman/rrna/gdnaisol.htm
Added: Tue May 14 2002, Hits: 7899, Reviews: 0 Write review Cached