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Protocols
- Concentration of Nucleic Acids by Ethanol Precipitation (The Minion Lab, College of Veterinary Medicine at Iowa State University)
The complete guide to concentration of nucleic acids including DNA, RNA, and oligos by ethanol precipitation. Also included is the method for isobutanol concentration of DNA.
http://mycoplasmas.vm.iastate.edu/lab_site/methods...
Added: Tue Nov 16 2004, Hits: 30716, Reviews: 0 Write review Cached - DNA extraction and precipitation
(Practical Approach Online, Oxford University Press)
Purification of DNA by phenol extraction and ethanol precipitation
http://www.oup.co.uk/pdf/pas/9v1-7-3.pdf
Added: Sat Aug 17 2002, Hits: 33505, Reviews: 0 Write review - DNA Precipitation (Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
Phenol/Chloroform extraction followed by ethanol precipitation
http://www-ufk.med.uni-rostock.de/main/hmueller/la...
Added: Sat Nov 02 2002, Hits: 8858, Reviews: 0 Write review Cached - Ethanol Precipitation of DNA (Kitto Lab, The University of Texas at Austin)
This procedure allows the concentration of DNA samples from dilute solution and the removal of unwanted salts from DNA samples.
http://research.cm.utexas.edu/bkitto/Kittolabpage/...
Added: Tue Oct 08 2002, Hits: 21137, Reviews: 0 Write review Cached -
Ethanol Precipitation of Nucleic Acids (Lab of Dr. Mark Barton Frank, Oklahoma Medical Research Foundation)
It gives great details on how ethanol precipitation works.
http://omrf.ouhsc.edu/~frank/ETOHPPT.html
Added: Thu Sep 01 2005, Hits: 13472, Reviews: 0 Write review Cached -
Ammonium acetate/ethanol precipition
(BBHelp)
http://www.uct.ac.za/depts/mmi/bbhelp/dna4.html#DN...
Added: Tue May 14 2002, Hits: 4401, Reviews: 0 Write review Cached -
Concentrating Nucleic Acids (DNA and RNA)
(R. A. Bowen, et al., Colorado State University)
The need to concentrate DNA or RNA from dilute, aqueous solution arises quite frequently, for example, in almost all protocols for purification of nucleic acids from cells. The method most commonly used for this purpose is precipitation with ethanol or isopropanol. This protocol gives many variations for precipitating DNA and RNA.
http://arbl.cvmbs.colostate.edu/hbooks/genetics/bi...
Added: Thu Sep 01 2005, Hits: 12442, Reviews: 0 Write review Cached -
Concentration of DNA by ethanol precipitation
(Bruce A. Roe)
http://www.genome.ou.edu/protocol_book/protocol_pa...
Added: Tue May 14 2002, Hits: 10220, Reviews: 0 Write review Cached -
Deproteination Using Phenol/Chloroform
(Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
Removes protein from nucleic acids
http://www.nottingham.ac.uk/~mbzspd/methods/Deprot...
Added: Sat Apr 10 2004, Hits: 3607, Reviews: 0 Write review Cached -
DNA Dialysis
(Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
DNA purification using molecularpourous membrane tubing
http://www-ufk.med.uni-rostock.de/main/hmueller/la...
Added: Sat Nov 02 2002, Hits: 1153, Reviews: 0 Write review Cached
Trouble Shootings and FAQs
- Why is isopropanol or ethanol used to precipitate nucleic acids?
- Precipitation of DNA from CsCl samples
- how to clean DNA>10KB?
- Useful tips on concentrating DNA
- What is a crystaline precipitate that I get when I precipitate my genomic DNA?
- Glycogen for DNA precipitation
- Tips on DNA precipitation using carriers
- How to dissolve DNA?
- Ethanol vs Isopropanol for DNA precipitation
- What's the function of NaAc in DNA precipitation?
- Isopropanol precipitation of DNA - best time and temperature?
- Tips on ethanol wash after nucleic acid precipitation
- Trouble resuspending DNA after EtOH precipitation