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Protocols
- DNA extraction and precipitation
(Practical Approach Online, Oxford University Press)
Purification of DNA by phenol extraction and ethanol precipitation
http://www.oup.co.uk/pdf/pas/9v1-7-3.pdf
Added: Sat Aug 17 2002, Hits: 40184, Reviews: 0 Write review - Ethanol Precipitation of DNA (Kitto Lab, The University of Texas at Austin)
This procedure allows the concentration of DNA samples from dilute solution and the removal of unwanted salts from DNA samples.
http://kitto.cm.utexas.edu/research/Kittolabpage/P...
Added: Tue Oct 08 2002, Hits: 22509, Reviews: 0 Write review Cached - DNA Precipitation (Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
Phenol/Chloroform extraction followed by ethanol precipitation
http://www-ufk.med.uni-rostock.de/lablinks/protoco...
Added: Sat Nov 02 2002, Hits: 1911, Reviews: 0 Write review Cached -
Ammonium acetate/ethanol precipition
(BBHelp)
http://www.uct.ac.za/depts/mmi/bbhelp/dna4.html#DN...
Added: Tue May 14 2002, Hits: 5904, Reviews: 0 Write review Cached -
Concentration of DNA by ethanol precipitation
(Bruce A. Roe)
http://www.genome.ou.edu/protocol_book/protocol_pa...
Added: Tue May 14 2002, Hits: 6966, Reviews: 0 Write review Cached -
Deproteination Using Phenol/Chloroform
(Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
Removes protein from nucleic acids
http://www.nottingham.ac.uk/~mbzspd/methods/Deprot...
Added: Sat Apr 10 2004, Hits: 323, Reviews: 0 Write review Cached -
DNA Dialysis
(Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
DNA purification using molecularpourous membrane tubing
http://www-ufk.med.uni-rostock.de/lablinks/protoco...
Added: Sat Nov 02 2002, Hits: 238, Reviews: 0 Write review Cached -
DNA Precipitation
(Gerard Lazo)
http://wheat.pw.usda.gov/~lazo/methods/lazo/dnappt...
Added: Tue May 14 2002, Hits: 3696, Reviews: 0 Write review Cached -
DNA Purification
(Gene Delivery Group, University of Birmingham)
If at A260/A280 the purity of DNA is out of the 1.8-2.0 range then the DNA should be purified to remove contaminants using Phenol/Chloroform/isopropanol
http://web.bham.ac.uk/can4psd4/brum/purify.html
Added: Mon Sep 09 2002, Hits: 883, Reviews: 0 Write review Cached -
DNA Purification and Precipitation
(John Roth, Division of Biological Sciences,University of California at Davis)
Using phenol and chroloform method
http://rothlab.ucdavis.edu/protocols/P31.html
Added: Tue May 25 2004, Hits: 6278, Reviews: 0 Write review Cached -
Ethanol Precipitation of DNA
(Julie B. Wolf, University of Maryland, Baltimore County)
http://www.research.umbc.edu/~jwolf/m5.htm#ethanol
Added: Tue May 14 2002, Hits: 5151, Reviews: 0 Write review Cached -
Fragment purification on Sephacryl S-500 spin columns
(Bruce A. Roe)
DNA fragments larger than a few hundred base pairs can be separated from smaller fragments by chromatography on a size exclusion column such as Sephacryl S-500.
http://www.genome.ou.edu/protocol_book/protocol_pa...
Added: Tue May 14 2002, Hits: 719, Reviews: 0 Write review Cached -
Improved Alcohol Precipitation of DNA
(John Roth, Division of Biological Sciences,University of California at Davis)
This method was suggested in a BRL "Focus" article several years ago (Zeugin & Hartley, 1985). It is a useful way to avoid coprecipitation of proteins and accumulation of salt in the final dried preparation.
http://rothlab.ucdavis.edu/protocols/Alcohol-Preci...
Added: Tue May 25 2004, Hits: 3145, Reviews: 0 Write review Cached -
Linear Acrylamide as Carrier for DNA Precipitation
(Janos Luka Ph.D., Department of Pathology, Eastern Virginia Medical School)
Linear polyacrylamide is an efficient carrier for precipitation of picogram amounts of nucleic acids with ethanol. It is cheap,easy to make and can be stored in refrigerator for several years. For complete reference of the method see: Ethanol precipitation of DNA with linear polyacrylamide as carrier.
http://herpesvirus.tripod.com/research/linearacryl...
Added: Fri Apr 16 2004, Hits: 1046, Reviews: 0 Write review Cached -
Linear Polyacrylamide as Carrier in Ethanol Precipitation of Nucleic Acids
(Hideaki Shiraishi, Kyoto University)
Linear polyacrylamide can be used as an efficient neutral carrier for precipitating nucleic acids with ethanol. Although glycogen is often used as carrier in ethanol precipitation, it has been shown that glycogen inhibits the activity of an ssDNA-binding protein on which linear polyacrylamide has no effect. Here is the method on how to prepare linear polyacrylamide used as carrier in ethanol precipitation of nucleic acids.
http://kuchem.kyoto-u.ac.jp/seika/shiraishi/protoc...
Added: Fri Jan 30 2004, Hits: 1529, Reviews: 0 Write review Cached - PCR clean up using 3M sodium acetate and chilled absolute ethanol (KAIMRC, NGHA )
Clean up PCR in 1.5 hrs. with a very easy steps.
Added: Sat Jan 02 2010, Reviews: 0 Write review -
Purification of PCR products
(Srel DNA Lab, University of Georgia)
PEG Cleaning of PCR products prior to sequencing
http://www.uga.edu/srel/DNA_Lab/PEG_Precip'00.rtf
Added: Mon Sep 23 2002, Hits: 1903, Reviews: 0 Write review Cached -
Purification of PCR products with Sephadex
(Vodkin Laboratory, University of Illinois)
http://soybeangenomics.cropsci.uiuc.edu/protocols/...
Added: Mon Aug 26 2002, Hits: 1132, Reviews: 0 Write review Cached -
Sephadex G-50 Column Purification of DNA
(Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
Spin column purification can be used to change buffers without a concomitant change in solution volume, to remove protein contaminants or to purify plasmid DNA suitable for sequencing.
http://www.nottingham.ac.uk/~mbzspd/methods/Sephad...
Added: Sat Apr 10 2004, Hits: 107, Reviews: 0 Write review Cached -
Sodium perchlorate/Isopropanol precipitation
(BBHelp)
http://www.uct.ac.za/depts/mmi/bbhelp/dna4.html#DN...
Added: Tue May 14 2002, Hits: 1395, Reviews: 0 Write review Cached