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New Forum Archives (2009-)
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General-Lab-Techniques
1.
Good Pipetting techniques -
(reply: 2)
2.
Gene expression profiling with small cell number -
(reply: 1)
3.
Isolation of protein and RNA from RNeasy Minikits? -
(reply: 5)
4.
Immunofluorescence: simultaneous polyclonal and monoclonal staining to same prot -
(reply: 1)
5.
Grass genomic DNA extraction -
(reply: 1)
6.
SDS page of acid soluble collagen -
(reply: 4)
7.
siRNA Agarose gel electrophoresis - Protocol -
(reply: 1)
8.
break disulfide bonds to study fluorescence -
(reply: 2)
9.
Looking for simple Papanicolaou (pap) staining protocol -
(reply: 2)
10.
Luminescence: Background or real signal? -
(reply: 2)
11.
Qubit 2.0 Fluorometer or NanoDrop( Lite) -
(reply: 2)
12.
Precautions for working with acridine orange? -
(reply: 1)
13.
Incubate tubes on ice vs in the refrigerator -
(reply: 2)
14.
Quick ImageJ question -
(reply: 3)
15.
Some primers dose not work -
(reply: 5)
16.
Problem with precipitating RNA from ethanol solution -
(reply: 1)
17.
clogging up qiagen filters -
(reply: 4)
18.
most common amino acid -
(reply: 3)
19.
Problem with Gamma H2AX foci staining -
(reply: 5)
20.
Pelleting bacterial cells -
(reply: 1)
21.
Inhibition of a protein upstream in signaling cascade ? -
(reply: 5)
22.
Proper western blotting -
(reply: 6)
23.
what is OD in terms of HPLC? -
(reply: 4)
24.
Sequencing RT-PCR product -
(reply: 3)
25.
Non-reproducible results/experiments -
(reply: 9)
26.
Low yield PCR product after gel purification -
(reply: 7)
27.
Slime formation in DNA extraction -
(reply: 12)
28.
Dilution question -
(reply: 3)
29.
Sodium Acetate Buffer -
(reply: 10)
30.
column chromatography -
(reply: 2)
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