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New Forum Archives (2009-)
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General-Lab-Techniques
1.
FLASH FREEZING AND ENZYME WORK INQUIRY -
(reply: 1)
2.
TBE BUFFER -
(reply: 2)
3.
OVERDILUTION OF TBE BUFFER -
(reply: 1)
4.
Homogenisation of protein -
(reply: 1)
5.
Can you stop a Chitin column after flow through? -
(reply: 1)
6.
Running a sucrose gradient -
(reply: 1)
7.
Denatured ethanol and DNA preservation -
(reply: 2)
8.
IPTG induced protein go to inclusion bodies -
(reply: 1)
9.
Timelapse microscopy and incubation (HEPES)? -
(reply: 1)
10.
pH rise upon filtration -
(reply: 2)
11.
Lifespan of HRP-conjugated Secondary antibody @ Room temperature? -
(reply: 2)
12.
cgh array -
(reply: 3)
13.
Whole blood storage and transport -
(reply: 5)
14.
urgent! calculating concentration of compound with different cell amounts -
(reply: 3)
15.
70% ethanol in tap water... -
(reply: 19)
16.
Making Phosphate buffer with calcium chloride -
(reply: 7)
17.
Culture HEK293T with poly-lysine -
(reply: 2)
18.
Can I autoclave Ammonium thiocyanate/Guanidine thiocyanate? -
(reply: 2)
19.
how to prepare TBE 10x -
(reply: 4)
20.
Southern blotting - high background, no clear signal? -
(reply: 4)
21.
Tracer study using 13C stable isotope. -
(reply: 1)
22.
Buffer-saturated Phenol -
(reply: 1)
23.
acrylamide information -
(reply: 1)
24.
transporting E coli on filter paper -
(reply: 5)
25.
Centrifugation - how to compensate lower g? -
(reply: 3)
26.
Percoll -
(reply: 1)
27.
Centrifuging-cells separation probs? -
(reply: 1)
28.
Interpreting Nanodrop results -
(reply: 2)
29.
Diluting antibody stocks in glycerol -
(reply: 8)
30.
what is the best way to increase my purified enzyme's concentration??? -
(reply: 1)
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