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Top : New Forum Archives (2009-): : General-Lab-Techniques
1. Lack of reproducibility using BCA to quantitate total protein - (reply: 4)
2. Agarose gel for determining RNA quality (image attached) - (reply: 3)
3. CRISPR-Cas9 System for In Vivo Genome Editing Experiments - (reply: 1)
4. Centrifuges in labs - (reply: 1)
5. Pre heat up and cooling on autoclave - (reply: 1)
6. Weird Immunofluorescence - (reply: 1)
7. McConkey agar - (reply: 2)
8. ELISA expiry dates/ bad results - (reply: 1)
9. Dilution calculation - (reply: 1)
10. Feedback to an alternative to Ethidium bromide - - (reply: 1)
11. Please help urgently - (reply: 2)
12. Please help urgently - (reply: 1)
13. How many people pellet bacteria cultures in their tissue culture centrifuge? - (reply: 4)
14. Claudin 3 Annealing Temperature - (reply: 1)
15. Diluting a stock - help please - (reply: 3)
16. To prepare 1M calcium chloride - (reply: 3)
17. Varying absorbance with varying volume in 384 well plate - (reply: 3)
18. Affinity Purification of 5′ Capped mRNA by Using High-Affinity GST-4E - (reply: 1)
19. Fluorescent and photobleach - (reply: 2)
20. Capillary electrophoresis separation - (reply: 2)
21. concentrate DNA - (reply: 3)
22. Media formulation with Insulin - (reply: 4)
23. Passaging cells - (reply: 1)
24. Lab report - plagiarism check - (reply: 4)
25. Quantification of IgG - (reply: 3)
26. Daily stimulation with cytokines - (reply: 1)
27. Trypan Blue if cells cultured in 96 well plate - (reply: 2)
28. cytokine reconstitution with fbs instead of bsa - (reply: 2)
29. solution - (reply: 7)
30. Tissue homogenization using Potassium phosphate buffer - (reply: 1)