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Electrophoresis
1.
agarose gel electrophoresis of RNA -
(reply: 1)
2.
EtBr in agarose gel and running buffer -
(reply: 3)
3.
15% SDS-PAGE issues -
(reply: 4)
4.
Protein of 460 kD on western blot -
(reply: 3)
5.
Help with bad electrophoresis run. -
(reply: 3)
6.
How to make gradient gel (10-20%), how to control the speed of flow? -
(reply: 4)
7.
Straight bands on DNA gel -
(reply: 3)
8.
Western blot- bands weak -
(reply: 10)
9.
western blotting troubleshooting -
(reply: 2)
10.
Application for calculating ELFO fragment length -
(reply: 1)
11.
Plasmides run higher than marker -
(reply: 4)
12.
Odd Bands on Gel -
(reply: 3)
13.
Agarose gell electrophoresis -
(reply: 1)
14.
Elchrom Origins -
(reply: 1)
15.
Pattern/cloud appearing on agarose gel -
(reply: 15)
16.
TBE buffer-what is the role of each component? -
(reply: 1)
17.
Problems with having to run the gels for so long -
(reply: 14)
18.
Ethidium Bromide Agarose Gel Hazard -
(reply: 6)
19.
Single Plasmid Gel Band -
(reply: 1)
20.
Buffer in Gel -
(reply: 6)
21.
agarose gel electrophoresis -
(reply: 5)
22.
Uneven bands denaturing agarose -
(reply: 2)
23.
What gel percent and Voltage is needed to separate 2680bp and 2600bp DNA nicely -
(reply: 7)
24.
Smear on agarose gel from QIAGEN extracted plant samples -
(reply: 1)
25.
E gels and the E gel opener -
(reply: 1)
26.
Why do we use sponges in the Western Transfer? -
(reply: 5)
27.
Non-hazardous substitute for ethidium bromide? -
(reply: 17)
28.
What causes surface fluorescence on agarose gels? -
(reply: 6)
29.
Problems with PAGE gels -
(reply: 1)
30.
carpet in gels for PCR products -
(reply: 2)
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