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Protocols
- Transformation (Gottschling Lab, Fred Hutchinson Cancer Research Center)
Including chemical transformation and lectroporation, preparation of competent cells for both methods.
http://labs.fhcrc.org/gottschling/Bacterial%20Prot...
Added: Fri Jul 26 2002, Hits: 2923, Reviews: 0 Write review Cached -
Antibiotic concentrations for E. coli selection
(Addgene.org)
Recommended antibiotic concentrations for LB media or agar plates.
http://www.addgene.org/pgvec1?f=v&cmd=showfile&fil...
Added: Thu Apr 29 2010, Hits: 586, Reviews: 0 Write review Cached -
Long Term Storage of Transformed E.coli
(Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
http://www-ufk.med.uni-rostock.de/lablinks/protoco...
Added: Sun Nov 03 2002, Hits: 256, Reviews: 0 Write review Cached -
Measuring Transformation Efficiency
(Pamela Stanley Lab Wiki, Yale University)
http://stanxterm.aecom.yu.edu/wiki/index.php?page=...
Added: Thu Mar 26 2009, Hits: 530, Reviews: 0 Write review Cached -
Transformation Of Bacteria By Heat Shock
(Pamela Stanley Lab Wiki, Yale University)
E.coli can be transformed using calcium, electroporation (best efficiency) or heat-shock. Competent cells for heat-shock based transformation can be easily prepared in the lab using PEG (polyethylene glycol), etc. but many get them from commercial vendors.
http://stanxterm.aecom.yu.edu/wiki/index.php?page=...
Added: Thu Mar 26 2009, Hits: 1621, Reviews: 0 Write review Cached