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Protocols

• Five-Minute Transformation (Chun-Ming Liu, )
  Quick E. Coli transformation method. It works!
  http://www.plant.dlo.nl/projects/hybtech/Liu/magic...
  Added: Tue May 14 2002, Hits: 2022, Reviews: 0  Write review  
• Large-scale transformation procedures (YGAC)
  This protocol merges a typical LiAc protocol with the reducing agent component of the One-step method. It gives >103/ ug efficiency for our strain. Recipe is for 800 transformations (90 mls cells).
  http://ygac.med.yale.edu/mtn/LS_transf_prot.stm
  Added: Tue May 14 2002, Hits: 430, Reviews: 0  Write review   Cached
• Standard Transformation Protocol (Promega)
  You can find general and useful protocol for the whole experiment of plasmid cloning from DNA and vector preparation, ligation, transformation, plasimd preparation and transfection...
  http://www.promega.com/tbs/tm033/tm033.html#iv0c
  Added: Tue May 14 2002, Hits: 3096, Reviews: 0  Write review   Cached
• Transformation (Lazo Lab)
  One-step PEG method
  http://wheat.pw.usda.gov/~lazo/methods/lazo/oneste...
  Added: Tue May 14 2002, Hits: 967, Reviews: 0  Write review   Cached
• Transformation of Competent Cells (RbCl2 Method) (Goldberg Lab)
  Very nice protocol for E. Coli transformation including competent cell preparation and recipes
  http://wheat.pw.usda.gov/~lazo/methods/goldberg/co...
  Added: Tue May 14 2002, Hits: 1334, Reviews: 0  Write review   Cached
• Transformation of E. coli (Hideaki Shiraishi, Kyoto University)
  Method based on H. Inoue, H. Nojima & H. Okayama, Gene 96, 23-28 (1990)
  http://kuchem.kyoto-u.ac.jp/seika/shiraishi/protoc...
  Added: Fri Jan 30 2004, Hits: 2091, Reviews: 0  Write review   Cached