Protocols
- Preparation of Electrocompetent Cells and Electroporation of E. coli
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
Detailed procedures
http://www.its.caltech.edu/%7Ebjorker/Prep_of_elec...
Added: Wed Aug 14 2002, Hits: 24347, Reviews: 0 Write review - Stable Transfection (Electroporation) (Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
Electroporation can be used for both transient and stable transfection of mammalian cells. Cells are placed in suspension in an appropriate electroporation buffer and put into an electroporation cuvette. DNA is added, the cuvette is connected to a power supply, and the cells are subjected to a high-voltage electrical pulse of defined magnitude and length. The cells are then allowed to recover briefly before they are placed in normal growth medium.
http://www-ufk.med.uni-rostock.de/lablinks/protoco...
Added: Sun Nov 03 2002, Hits: 698, Reviews: 0 Write review Cached - Transformation of E. coli by Electroporation (Kitto Lab, The University of Texas at Austin)
This procedure prepares glycerol stock cultures of bacteria for electroporative transformation.
http://kitto.cm.utexas.edu/research/Kittolabpage/P...
Added: Tue Oct 08 2002, Hits: 12300, Reviews: 0 Write review Cached -
Electroporation of P. Aeruginosa
(Goldberg Lab)
Detailed protocol for preparing electroporation cell and electroporation procedure.
http://wheat.pw.usda.gov/~lazo/methods/goldberg/el...
Added: Tue May 14 2002, Hits: 842, Reviews: 0 Write review Cached -
Preparation of Electrocompetent Bacteria
(Steven Finkbeiner, UCSF)
http://www.gladstone.ucsf.edu/gladstone/files/fink...
Added: Fri Feb 20 2009, Hits: 6036, Reviews: 0 Write review -
Transformation of E. coli by Electroporation
(Julie B. Wolf, University of Maryland, Baltimore County)
provides details for preparing cell and DNA and electroporation procedure
http://www.research.umbc.edu/~jwolf/m7.htm
Added: Tue May 14 2002, Hits: 5639, Reviews: 0 Write review Cached -
Transformation of E. Coli by Electroporation
(Goldberg Lab)
Very nice protocol for electroporation including cell preparation and recipes
http://wheat.pw.usda.gov/~lazo/methods/goldberg/el...
Added: Tue May 14 2002, Hits: 2196, Reviews: 0 Write review Cached -
Transformation of E. coli by Electroporation
(Kitto Lab, The University of Texas at Austin (updated))
This procedure prepares glycerol stock cultures of bacteria for electroporative transformation.
http://kitto.cm.utexas.edu/research/Kittolabpage/P...
Added: Sat Mar 14 2009, Hits: 2894, Reviews: 0 Write review Cached