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E.coli long term storage

Long Term Storage of Transformed E.coli

  1. Transfer 10 ml of 250 ml overnight culture in sterile flip-cap 15 ml Tube.
  2. Add sterile Glycerol to 15 % final conc. (i.e. 1.875 ml of 80% Glycerine stock solution).
  3. Store at -80°C.