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Protocols
- How to Make Competent Cells (Sosnick Lab, University of Chicago)
This 6-step protocol describes the CaCl2 method for making competent cells.
http://sosnick.uchicago.edu/competent_cells.html
Added: Tue Aug 13 2002, Hits: 29619, Reviews: 0 Write review Cached - Preparation of Electrocompetent Cells and Electroporation of E. coli
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
Detailed procedures
http://www.its.caltech.edu/%7Ebjorker/Prep_of_elec...
Added: Wed Aug 14 2002, Hits: 20805, Reviews: 1 Read reviews Write review - Competent Cell prepration--A modified method
(Protocol Online)
A simple and best method for preparation of competent cells
Added: Sun Sep 14 2008, Reviews: 0 Write review -
Cool Growth of Competent E . coli Cells
(Nonet Lab - Washington University School of Medicine)
This protocol is very effective and simple. The fact that the cells are grown at low temperature greatly increase the OD range during which the cells will prep up at high efficiency.
http://thalamus.wustl.edu/nonetlab/ResourcesF/comp...
Added: Sat Nov 01 2003, Hits: 7142, Reviews: 0 Write review Cached -
Electroporation Competent E.Coli Preparation
(Bowtell Lab)
Including procedures for cell preparation and electroporation
http://grimwade.biochem.unimelb.edu.au/bowtell/mol...
Added: Tue May 14 2002, Hits: 3507, Reviews: 0 Write review Cached -
Frozen Competent E. coli Cells
(Koshland Lab, Carnegie Institution of Washington/Howard Hughes Medical Institute)
How to preserve and reuse your competent cells.
http://www.ciwemb.edu/labs/koshland/Protocols/BACT...
Added: Thu Jun 24 2004, Hits: 9222, Reviews: 0 Write review Cached -
Preparation of Chemical Competent Cells
(A. Untergasser)
A protocol for highly chemicaly competent bacteria.
http://www.untergasser.de/lab/protocols/competent_...
Added: Sun Nov 16 2008, Hits: 126, Reviews: 0 Write review -
Preparation of Competent Cell (Inoue "ultra-competent" method)
( )
) (NWFSC)
http://micro.nwfsc.noaa.gov/protocols/inoue.html
Added: Tue May 14 2002, Hits: 8457, Reviews: 0 Write review Cached -
Preparation of Competent Cell (Ubidium Chloride Method)
(NWFSC)
http://micro.nwfsc.noaa.gov/protocols/rbcl.html
Added: Tue May 14 2002, Hits: 4257, Reviews: 1 Read reviews Write review Cached -
Preparation of Competent Cells
(BBHelp)
This protocol describes both CaCl2 and DMSO methods for preparing competent cells.
http://web.uct.ac.za/depts/mmi/bbhelp/bac1.html
Added: Tue May 14 2002, Hits: 13734, Reviews: 0 Write review Cached