Protocols
- Recovering Plasmids from Stab Culture (Addgene.org)
Isolating a Single Colony
Recovering plasmid DNA
Making glycerol stock for long-term storage
Recipes
http://www.addgene.org/pgvec1?f=v&cmd=showfile&fil...
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Bacterial Glycerol Stock
(UAB)
http://homepage.uab.edu/wzhang/BacterialGlycerolSt...
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Long Term Storage of Bacterial Strains
(Donis Keller Lab)
Bacterial strains may be stored indefinitely at low temperatures (- 20 degrees C and -80 degrees C) in 15 to 40% glycerol.
http://hg.wustl.edu/hdk_lab_manual/plasmid/plsmid0...
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Making a long term stock of bacteria
(OpenWetWare)
http://openwetware.org/wiki/Endy:Making_a_long_ter...
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Retrieving a Registry glycerolBacterial Glycerol Stocks
(Wolfson Centre for Applied Structural Biology)
http://wolfson.huji.ac.il/expression/procedures/ba...
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Streaking Bacteria for Single Colonies
(John Roth's laboratory, UC Davis)
http://rothlab.ucdavis.edu/protocols/M01.html
Added: Wed Oct 16 2002, Hits: 5422, Reviews: 0 Write review Cached -
Streaking Bacterial Stocks
(Donis Keller Lab)
Bacterial cells are streaked onto a medium to obtain an independent isolate. This is done to reduce the likelihood of working with a culture which has become contaminated and/or has accumulated mutations.
http://hg.wustl.edu/hdk_lab_manual/plasmid/plsmid0...
Added: Tue May 14 2002, Hits: 2912, Reviews: 0 Write review Cached