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Protocols

• Recovering Plasmids from Stab Culture (Addgene.org)
  Isolating a Single Colony
  Recovering plasmid DNA
  Making glycerol stock for long-term storage
  Recipes
  
  http://www.addgene.org/pgvec1?f=v&cmd=showfile&fil...
  Added: Thu Apr 29 2010, Hits: 302, Reviews: 0  Write review   Cached
• Bacterial Glycerol Stock (UAB)
  http://homepage.uab.edu/wzhang/BacterialGlycerolSt...
  Added: Thu Apr 29 2010, Hits: 963, Reviews: 0  Write review   Cached
• Long Term Storage of Bacterial Strains (Donis Keller Lab)
  Bacterial strains may be stored indefinitely at low temperatures (- 20 degrees C and -80 degrees C) in 15 to 40% glycerol.
  http://hg.wustl.edu/hdk_lab_manual/plasmid/plsmid0...
  Added: Tue May 14 2002, Hits: 2275, Reviews: 0  Write review   Cached
• Making a long term stock of bacteria (OpenWetWare)
  http://openwetware.org/wiki/Endy:Making_a_long_ter...
  Added: Thu Apr 29 2010, Hits: 412, Reviews: 0  Write review   Cached
• Retrieving a Registry glycerolBacterial Glycerol Stocks (Wolfson Centre for Applied Structural Biology)
  http://wolfson.huji.ac.il/expression/procedures/ba...
  Added: Thu Apr 29 2010, Hits: 194, Reviews: 0  Write review   Cached
• Streaking Bacteria for Single Colonies (John Roth's laboratory, UC Davis)
  http://rothlab.ucdavis.edu/protocols/M01.html
  Added: Wed Oct 16 2002, Hits: 3532, Reviews: 0  Write review   Cached
• Streaking Bacterial Stocks (Donis Keller Lab)
  Bacterial cells are streaked onto a medium to obtain an independent isolate. This is done to reduce the likelihood of working with a culture which has become contaminated and/or has accumulated mutations.
  http://hg.wustl.edu/hdk_lab_manual/plasmid/plsmid0...
  Added: Tue May 14 2002, Hits: 1593, Reviews: 0  Write review   Cached