Protocols
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Bacteria Storage
(Gerard R. Lazo)
Preserve E. coli strains in stab agar or glycerol for mid-term storage and lyophilize for long-term storage.
http://wheat.pw.usda.gov/~lazo/methods/lazo/bactst...
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Bacterial Colony PCR
(Karl Clark)
Allows quick identification of correct clones if primers exist that allow clone identification by size or hybridization.
http://www.cbs.umn.edu/~kclark/protocols/bactPCR.h...
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Screen of Recombinant
(Dr. Chen, Dept of Biochem. & Mol. Biology, Univ. College London)
Four methods are introduced including clony PCR, boiling ..
http://www.biochem.ucl.ac.uk/bsm/nmr/protocols/pro...
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Screening clone by PCR
(Herskowitz Lab)
Add colony directly into PCR tubes without plasmid preparation
http://www.sacs.ucsf.edu/home/HerskowitzLab/protoc...
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Toothpick Plasmid Assay
(Donis Keller Lab)
The toothpick assay is used to identify bacterial colonies containing recombinant plasmids following transformation and to obtain an approximation of the plasmid insert sizes.
http://humgen.wustl.edu/hdk_lab_manual/plasmid/pls...
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Transfer of DNA from Bacterial Colonies to Nylon Membrane or Nitrocellulose
(Donis Keller Lab)
To transfer DNA from bacterial colonies to nylon (or nitrocellulose) membrane so that a library can be screened by hybridization.
http://humgen.wustl.edu/hdk_lab_manual/plasmid/pls...
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