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91. Cell toxicity and color - (reply: 1)
92. Leaky Expression? - (reply: 5)
93. Why a previously soluble protein is now going into inclusion bodies? - protein expression (reply: 4)
94. BL21 Plasmid extraction? - (reply: 4)
95. Flag-IP elution with TEV protease - (reply: 1)
96. Purification Optimisation - (reply: 4)
97. protein expresses using pGEX4T-3 but not being purified? - trapped in GST (reply: 8)
98. Membrane protein - (reply: 11)
99. No Ni-NTA binding--internal start codon of NdeI in pET vector? - (reply: 1)
100. cysteine-rich protein - polydnavirus (reply: 2)
101. Immunoaffinity chromatography - (reply: 7)
102. Growing E.coli cells - (reply: 1)
103. IPTG induction - at what cell density (reply: 5)
104. protein extraction from Daphnia/rotifera - (reply: 3)
105. Recombinant protein solution turns yellow when concentrated - (reply: 3)
106. Cell lysis method for running cell lysate on Western blot - (reply: 4)
107. purification failed - how to restart? - Protein diluted in many fractions (reply: 7)
108. pH and Ni-column? - (reply: 1)
109. Looking for high-capacity HPLC column - (reply: 2)
110. Problems with purifying a secret His-protein expressed in plant - (reply: 3)
111. No protein expression in pKK223-3 vector in JM105 - (reply: 3)
112. Transfection of 293-T cells in 6-well plate, using PEI - (reply: 4)
113. GST tag protein purification - (reply: 2)
114. Problem with His Tag purification - using Promega HisLink spin purification kit (reply: 1)
115. do i need to desalt my protein after purification - (reply: 1)
116. Inclusion body Idea! Tell me what you think! - (reply: 10)
117. GST expression problem - low fusion, high GST alone (reply: 3)
118. Acetone precipitation - (reply: 2)
119. Purifying protein with no his tag - (reply: 5)
120. large quantity of high concentration protein needed - (reply: 3)
92. Leaky Expression? - (reply: 5)
93. Why a previously soluble protein is now going into inclusion bodies? - protein expression (reply: 4)
94. BL21 Plasmid extraction? - (reply: 4)
95. Flag-IP elution with TEV protease - (reply: 1)
96. Purification Optimisation - (reply: 4)
97. protein expresses using pGEX4T-3 but not being purified? - trapped in GST (reply: 8)
98. Membrane protein - (reply: 11)
99. No Ni-NTA binding--internal start codon of NdeI in pET vector? - (reply: 1)
100. cysteine-rich protein - polydnavirus (reply: 2)
101. Immunoaffinity chromatography - (reply: 7)
102. Growing E.coli cells - (reply: 1)
103. IPTG induction - at what cell density (reply: 5)
104. protein extraction from Daphnia/rotifera - (reply: 3)
105. Recombinant protein solution turns yellow when concentrated - (reply: 3)
106. Cell lysis method for running cell lysate on Western blot - (reply: 4)
107. purification failed - how to restart? - Protein diluted in many fractions (reply: 7)
108. pH and Ni-column? - (reply: 1)
109. Looking for high-capacity HPLC column - (reply: 2)
110. Problems with purifying a secret His-protein expressed in plant - (reply: 3)
111. No protein expression in pKK223-3 vector in JM105 - (reply: 3)
112. Transfection of 293-T cells in 6-well plate, using PEI - (reply: 4)
113. GST tag protein purification - (reply: 2)
114. Problem with His Tag purification - using Promega HisLink spin purification kit (reply: 1)
115. do i need to desalt my protein after purification - (reply: 1)
116. Inclusion body Idea! Tell me what you think! - (reply: 10)
117. GST expression problem - low fusion, high GST alone (reply: 3)
118. Acetone precipitation - (reply: 2)
119. Purifying protein with no his tag - (reply: 5)
120. large quantity of high concentration protein needed - (reply: 3)