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61. protein precipitation after enterokinase cleavage - (reply: 1)
62. expression by hsp60 in E.coli - (reply: 3)
63. Help:how to get better resolution using FPLC? - (reply: 4)
64. Problems with cell lysis - (reply: 3)
65. How to induce expression in mammalian cells with the pcDNA/V5/GW vector ? - (reply: 3)
66. pichia expression - (reply: 2)
67. protein colour problem - (reply: 2)
68. dialysis - (reply: 2)
69. Inclusion bodies protein determination - (reply: 1)
70. Lack of Expression in pQE30 clone - (reply: 8)
71. GST or His tag for protein purification? - Tag choise + native vs denaturing conditions (reply: 2)
72. wrong protein size - (reply: 6)
73. protein extraction from yeast--all extracted proteins are below 50KD - (reply: 1)
74. Sephadex - (reply: 4)
75. TAT-GFP expression from pRSET vector - (reply: 1)
76. proteolytic cleavage - proteolytic cleavage (reply: 1)
77. Protease Inhibitors? - I want to know what are the best protease inhibitors to yeast. (reply: 3)
78. IP with Monoclonal Antibodies - (reply: 1)
79. measuring Isoelectric point - (reply: 2)
80. Ni-TED vs Ni-NTA - His-Tag purification (reply: 4)
81. Concentrating protein - (reply: 5)
82. Nanodrop - (reply: 3)
83. Problem purifying GST-fusion expressed in Baculovirus - (reply: 1)
84. Any experience with "Strep tag" fusion peptides & Strep-Tactin pur - (reply: 1)
85. protein inclusion bodies.. purpose of multiple water washes after lysate centrif - (reply: 5)
86. Batch or mixing method? - (reply: 1)
87. cannot find the expression protein - (reply: 5)
88. How can I increase protein expression in S. cerevisiae? - (reply: 6)
89. protein purification from yeast - (reply: 2)
90. Problem in concentrating protein - (reply: 1)
62. expression by hsp60 in E.coli - (reply: 3)
63. Help:how to get better resolution using FPLC? - (reply: 4)
64. Problems with cell lysis - (reply: 3)
65. How to induce expression in mammalian cells with the pcDNA/V5/GW vector ? - (reply: 3)
66. pichia expression - (reply: 2)
67. protein colour problem - (reply: 2)
68. dialysis - (reply: 2)
69. Inclusion bodies protein determination - (reply: 1)
70. Lack of Expression in pQE30 clone - (reply: 8)
71. GST or His tag for protein purification? - Tag choise + native vs denaturing conditions (reply: 2)
72. wrong protein size - (reply: 6)
73. protein extraction from yeast--all extracted proteins are below 50KD - (reply: 1)
74. Sephadex - (reply: 4)
75. TAT-GFP expression from pRSET vector - (reply: 1)
76. proteolytic cleavage - proteolytic cleavage (reply: 1)
77. Protease Inhibitors? - I want to know what are the best protease inhibitors to yeast. (reply: 3)
78. IP with Monoclonal Antibodies - (reply: 1)
79. measuring Isoelectric point - (reply: 2)
80. Ni-TED vs Ni-NTA - His-Tag purification (reply: 4)
81. Concentrating protein - (reply: 5)
82. Nanodrop - (reply: 3)
83. Problem purifying GST-fusion expressed in Baculovirus - (reply: 1)
84. Any experience with "Strep tag" fusion peptides & Strep-Tactin pur - (reply: 1)
85. protein inclusion bodies.. purpose of multiple water washes after lysate centrif - (reply: 5)
86. Batch or mixing method? - (reply: 1)
87. cannot find the expression protein - (reply: 5)
88. How can I increase protein expression in S. cerevisiae? - (reply: 6)
89. protein purification from yeast - (reply: 2)
90. Problem in concentrating protein - (reply: 1)