Acetone precipitation - (Mar/25/2008 )
I got struggle with the dissolving my pellet after acetone precipitation with PBS as a buffer of choice.
do you think i need to change the buffer, but i need to be compatible with iTRAQ or any subsequent analysis!
also, how much do you think we need to add to the pellet!!
thanking in advance
Is your pellet completely dry? Any residual acetone can make resuspension very very hard. let it dry for an hr or more.
first of all many thanks for your replay. I thought I'll never get an answer but you keep the hope up.
However, someone told that keeping dry too much will be very hard to resuspension again!!
also, do you recommend to use TCA and when ! by the way, I'm propose to use for iTRAQ.
again Many thanks for your helping...