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121. continuous glycerol gradient - (reply: 2)
122. Cloned genes dont express! - (reply: 6)
123. how to refold insoluble protein after purification - (reply: 10)
124. Do I need to clone into gateway vector or pGEMT easy vector before cloning into - (reply: 1)
125. how to express GST fusion trunctated protein? - GST fusion protein (reply: 3)
126. Ion exchange media help - TEAE-cellulose & Dowex 1 (reply: 2)
127. dry Ni-NTA resin - can it be reconstituted? (reply: 1)
128. Alpha-Amylase expression and purification - (reply: 3)
129. pectinase precipitation - how to precipitate (reply: 2)
130. Purification Rookie - advice appreciated - (reply: 2)
131. Get rib of albumin - (reply: 4)
132. Some basic questions about cell fractionation and incl.bodies - (reply: 2)
133. What happens when we don't wash the Ni-NTA column? - (reply: 2)
134. Protein expression in Gram(-) bacteria - Protein expression by using special S-D sequence (reply: 1)
135. ATG's start codon - (reply: 1)
136. pull down troubles - (reply: 1)
137. getting rid of protein above 60 kDa - (reply: 7)
138. Concentrating recombinant protein without dialysis - (reply: 5)
139. NO EXPRESSION IN LARGE SCALE - (reply: 2)
140. Any good method for resuspension protein from TCA/acetone precip ? - (reply: 4)
141. GST-fusion won't elute! - (reply: 8)
142. Protein expression help needed - Mammalian cells (reply: 11)
143. inductions - inductions for expression of protiens (reply: 1)
144. Size Exclusion Column - Proteins not coming out ! - BMP-2 (30 kDa) is inhjected in a colum (size exclusion at 100kDa) and (reply: 2)
145. His tagged protein purification - (reply: 2)
146. Elution from PD-10 column - (reply: 11)
147. protein expression in Pichia pastoris - (reply: 90)
148. His tag protein purification problem - (reply: 65)
149. problem with amonium sulfate precipitation - What should i do ? (reply: 25)
122. Cloned genes dont express! - (reply: 6)
123. how to refold insoluble protein after purification - (reply: 10)
124. Do I need to clone into gateway vector or pGEMT easy vector before cloning into - (reply: 1)
125. how to express GST fusion trunctated protein? - GST fusion protein (reply: 3)
126. Ion exchange media help - TEAE-cellulose & Dowex 1 (reply: 2)
127. dry Ni-NTA resin - can it be reconstituted? (reply: 1)
128. Alpha-Amylase expression and purification - (reply: 3)
129. pectinase precipitation - how to precipitate (reply: 2)
130. Purification Rookie - advice appreciated - (reply: 2)
131. Get rib of albumin - (reply: 4)
132. Some basic questions about cell fractionation and incl.bodies - (reply: 2)
133. What happens when we don't wash the Ni-NTA column? - (reply: 2)
134. Protein expression in Gram(-) bacteria - Protein expression by using special S-D sequence (reply: 1)
135. ATG's start codon - (reply: 1)
136. pull down troubles - (reply: 1)
137. getting rid of protein above 60 kDa - (reply: 7)
138. Concentrating recombinant protein without dialysis - (reply: 5)
139. NO EXPRESSION IN LARGE SCALE - (reply: 2)
140. Any good method for resuspension protein from TCA/acetone precip ? - (reply: 4)
141. GST-fusion won't elute! - (reply: 8)
142. Protein expression help needed - Mammalian cells (reply: 11)
143. inductions - inductions for expression of protiens (reply: 1)
144. Size Exclusion Column - Proteins not coming out ! - BMP-2 (30 kDa) is inhjected in a colum (size exclusion at 100kDa) and (reply: 2)
145. His tagged protein purification - (reply: 2)
146. Elution from PD-10 column - (reply: 11)
147. protein expression in Pichia pastoris - (reply: 90)
148. His tag protein purification problem - (reply: 65)
149. problem with amonium sulfate precipitation - What should i do ? (reply: 25)