TUNEL Staining - non-fluorescent (Nov/29/2007 )
1st Q: i want to change my TUNEL staining method (In situ Cell Detection Kit, Fluorescein). does anyone has experience with TUNEL stainings which i can check in light-microscope?
2nd Q: is it possible that macrophages and other leukocytes (infiltrates) are prone to false positive TUNEL stains in tissue sections?
thanks for supportive replies
not sure we are looking for the same thing but i'd trust (cleaved) caspase 3 over tunel any day
I was told that TUNEL staining can give false positive results in general.
Ideed. We used here in our lab to analyse T cells coincubated with tumor cells and the T cells were also tunel positive (not expected).
We think that maybe because the T cells are prone to recombination (T cell receptor), they have chromosome breaches and then
are also stained.