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His-tag Related Discussions
  1. Re-purification of His-tagged protein over IMAC - (reply: 2)
  2. Designing primers with his-tag for pET28a+ vector - (reply: 2)
  3. GST fusion protein - two dominant bands - (reply: 3)
  4. Nonspecific proteins eluting with HIS-tagged peptide - (reply: 4)
  5. His-tag protein not binding to resin - (reply: 9)
  6. Difficulty eluting His-tag Zn metalloprotease - (reply: 1)
  7. Collagen binding protein expression E. coli His-tag - (reply: 1)
  8. Making a fusion protein - (reply: 1)
  9. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
  10. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
  11. Troubleshooting: His-Tag Nickle Column Purification - (reply: 1)
  12. how do you inactivate His-tag affinity beads?? - (reply: 4)
  13. his-tag purification - (reply: 9)
  14. His-tag and GST-tag - (reply: 1)
  15. designing fusion protein with Flag tag - (reply: 1)
  16. Flag fusion protein not detected by Flag antibody - (reply: 8)
  17. Luciferase fusion protein? - (reply: 1)
  18. Anyone has experience in using estrogen receptor (ER) fusion protein? - (reply: 1)
  19. trouble in his-tagged protein binding with Ni-NTA agarose - (reply: 3)
  20. Trouble in GST- fusion protein purification - (reply: 1)
  21. Why GFP gets cutted from my fusion protein? - (reply: 9)
  22. GST fusion protein purification - (reply: 2)
  23. his-tag prufication lysis problem:( - (reply: 4)
  24. co-eluted protein with his-tag protein - (reply: 1)
  25. Help with His-Tag protein purification - (reply: 2)
  26. GFP fusion protein not expressing! - (reply: 2)
  27. Nickel IMAC for His-tag - column goes dark brown! What does this mean? - (reply: 6)
  28. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
  29. How to transfer His-tag from N- to C-terminus in plasmid - (reply: 1)
  30. What's the difference between megnatic beads and agarose beads to IP His-tag - (reply: 3)
  31. Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
  32. Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
  33. Difficulty in expressing fusion protein - (reply: 1)
  34. what is the molecular weight of fusion protein in pET-32a - molecular weight of - (reply: 1)
  35. Problems eluting a GST fusion protein in GSTrap FF - (reply: 6)
  36. Fc fusion protein smearing on SDS PAGE - (reply: 2)
  37. Help with small protein with his-tag purification - (reply: 7)
  38. Help with protein expression - how to optimize expression of large GST fusion protein (reply: 4)
  39. HIS-tagged protein purification - (reply: 12)
  40. His-tagged protein purification problem - (reply: 2)
  41. His-tag protein vs. BSA - (reply: 3)
  42. insoluble fusion protein - (reply: 1)
  43. Fusion protein linker - (reply: 2)
  44. His-Tag protein purification:protein eluted with NaOH??? - (reply: 3)
  45. His-tag purification post Enterokinase digestion - Buffer exchange? (reply: 1)
  46. Fusion Protein - (reply: 3)
  47. His-tag protein purification - aggregation of protein (reply: 5)
  48. Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
  49. His-tagged protein purification - (reply: 3)
  50. Designing primer to remove his-tag - (reply: 1)
  51. His-tag proteins not eluted from Ni-Beads - (reply: 2)
  52. blocking the resin for his-tag pull down - what kind of blocking reagents (reply: 3)
  53. amount of plasmid for transformation and fusion protein expression - (reply: 1)
  54. gst fusion protein - (reply: 3)
  55. His-tag protein expression - (reply: 1)
  56. His-tag protein lost it's tag - (reply: 2)
  57. GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
  58. Protein at low PH - Strange behavior of His-tagged protein at low PH (reply: 1)
  59. Antibody for Fusion Protein - Antibody/ies against fusion protein (reply: 2)
  60. More His-tag problems - (reply: 4)
  61. expression of His-tagged protein in insect cells - (reply: 2)
  62. Contamination in GST fusion protein purification - (reply: 2)
  63. His-tagged protein runs much faster than expected - 6His-Tagged CcpA protein runs around 26 kD whereas I expect to run 37 (reply: 7)
  64. problem in the GST fusion protein purification - (reply: 4)
  65. Role of imidazole in lysis buffer - for obtaining recombinant his-tag protein (reply: 4)
  66. fusion protein construction - (reply: 1)
  67. How do I get rid of this contaminant? - His-tagged protein eluted with 300mM imidazole (reply: 13)
  68. How to check the translation? - His-tagged protein expression (reply: 8)
  69. dnase treatment before his-tag purification - (reply: 3)
  70. Problems in His-tag protein purification with TALON - (reply: 1)
  71. GST fusion protein purification - (reply: 1)
  72. B-per GST fusion protein purification kit - (reply: 2)