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His-tag Related Discussions
  1. GST fusion protein - two dominant bands - (reply: 3)
  2. Nonspecific proteins eluting with HIS-tagged peptide - (reply: 4)
  3. His-tag protein not binding to resin - (reply: 9)
  4. Difficulty eluting His-tag Zn metalloprotease - (reply: 1)
  5. Collagen binding protein expression E. coli His-tag - (reply: 1)
  6. Making a fusion protein - (reply: 1)
  7. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
  8. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
  9. Troubleshooting: His-Tag Nickle Column Purification - (reply: 1)
  10. how do you inactivate His-tag affinity beads?? - (reply: 4)
  11. his-tag purification - (reply: 9)
  12. His-tag and GST-tag - (reply: 1)
  13. designing fusion protein with Flag tag - (reply: 1)
  14. Flag fusion protein not detected by Flag antibody - (reply: 8)
  15. Luciferase fusion protein? - (reply: 1)
  16. Anyone has experience in using estrogen receptor (ER) fusion protein? - (reply: 1)
  17. trouble in his-tagged protein binding with Ni-NTA agarose - (reply: 3)
  18. Trouble in GST- fusion protein purification - (reply: 1)
  19. Why GFP gets cutted from my fusion protein? - (reply: 9)
  20. GST fusion protein purification - (reply: 2)
  21. his-tag prufication lysis problem:( - (reply: 4)
  22. co-eluted protein with his-tag protein - (reply: 1)
  23. Help with His-Tag protein purification - (reply: 2)
  24. GFP fusion protein not expressing! - (reply: 2)
  25. Nickel IMAC for His-tag - column goes dark brown! What does this mean? - (reply: 6)
  26. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
  27. How to transfer His-tag from N- to C-terminus in plasmid - (reply: 1)
  28. What's the difference between megnatic beads and agarose beads to IP His-tag - (reply: 3)
  29. Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
  30. Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
  31. Difficulty in expressing fusion protein - (reply: 1)
  32. what is the molecular weight of fusion protein in pET-32a - molecular weight of - (reply: 1)
  33. Problems eluting a GST fusion protein in GSTrap FF - (reply: 6)
  34. Fc fusion protein smearing on SDS PAGE - (reply: 2)
  35. Help with small protein with his-tag purification - (reply: 7)
  36. Help with protein expression - how to optimize expression of large GST fusion protein (reply: 4)
  37. HIS-tagged protein purification - (reply: 12)
  38. His-tagged protein purification problem - (reply: 2)
  39. His-tag protein vs. BSA - (reply: 3)
  40. insoluble fusion protein - (reply: 1)
  41. Fusion protein linker - (reply: 2)
  42. His-Tag protein purification:protein eluted with NaOH??? - (reply: 3)
  43. His-tag purification post Enterokinase digestion - Buffer exchange? (reply: 1)
  44. Fusion Protein - (reply: 3)
  45. His-tag protein purification - aggregation of protein (reply: 5)
  46. Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
  47. His-tagged protein purification - (reply: 3)
  48. Designing primer to remove his-tag - (reply: 1)
  49. His-tag proteins not eluted from Ni-Beads - (reply: 2)
  50. blocking the resin for his-tag pull down - what kind of blocking reagents (reply: 3)
  51. amount of plasmid for transformation and fusion protein expression - (reply: 1)
  52. gst fusion protein - (reply: 3)
  53. His-tag protein expression - (reply: 1)
  54. His-tag protein lost it's tag - (reply: 2)
  55. GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
  56. Protein at low PH - Strange behavior of His-tagged protein at low PH (reply: 1)
  57. Antibody for Fusion Protein - Antibody/ies against fusion protein (reply: 2)
  58. More His-tag problems - (reply: 4)
  59. expression of His-tagged protein in insect cells - (reply: 2)
  60. Contamination in GST fusion protein purification - (reply: 2)
  61. His-tagged protein runs much faster than expected - 6His-Tagged CcpA protein runs around 26 kD whereas I expect to run 37 (reply: 7)
  62. problem in the GST fusion protein purification - (reply: 4)
  63. Role of imidazole in lysis buffer - for obtaining recombinant his-tag protein (reply: 4)
  64. fusion protein construction - (reply: 1)
  65. How do I get rid of this contaminant? - His-tagged protein eluted with 300mM imidazole (reply: 13)
  66. How to check the translation? - His-tagged protein expression (reply: 8)
  67. dnase treatment before his-tag purification - (reply: 3)
  68. Problems in His-tag protein purification with TALON - (reply: 1)
  69. GST fusion protein purification - (reply: 1)
  70. B-per GST fusion protein purification kit - (reply: 2)