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GST-tag Related Discussions
  1. GST fusion protein - two dominant bands - (reply: 2)
  2. Thrombin cleaved GST-fusion protein- multiple bands - (reply: 2)
  3. Beads pull down only free GST - (reply: 14)
  4. Making a fusion protein - (reply: 1)
  5. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
  6. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
  7. His-tag and GST-tag - (reply: 1)
  8. designing fusion protein with Flag tag - (reply: 1)
  9. Flag fusion protein not detected by Flag antibody - (reply: 8)
  10. Luciferase fusion protein? - (reply: 1)
  11. GST tag fusion protein--- help please - (reply: 4)
  12. Anyone has experience in using estrogen receptor (ER) fusion protein? - (reply: 1)
  13. Difficulty in Expression of Protein Having 73% GC contents in GST Tag Vectrors - (reply: 3)
  14. GST pull down assay: GST binds to the prey protein. Negative control shows bindi - (reply: 4)
  15. Soluble protien of GST - (reply: 2)
  16. Cleavage of GST-tag with Factor Xa - (reply: 1)
  17. Trouble in GST- fusion protein purification - (reply: 1)
  18. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
  19. C-terminal GST fusion problem in mammalian cell - (reply: 6)
  20. minimum recombinant protein amounts for gst pulldown - (reply: 4)
  21. GST TAG - (reply: 3)
  22. Residues on the N terminal GST Tag - (reply: 1)
  23. Peptide recuperation after FactorXa cleavage using GST fusion - (reply: 1)
  24. Why GFP gets cutted from my fusion protein? - (reply: 9)
  25. GST fusion protein purification - (reply: 2)
  26. Could anyone help me out with the removal GST tag from my recombinant protein? - (reply: 1)
  27. GFP fusion protein not expressing! - (reply: 2)
  28. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
  29. GST tag protein- soluble or insoluble??? help! - (reply: 2)
  30. Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
  31. Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
  32. Difficulty in expressing fusion protein - (reply: 1)
  33. what is the molecular weight of fusion protein in pET-32a - molecular weight of - (reply: 1)
  34. Problems eluting a GST fusion protein in GSTrap FF - (reply: 6)
  35. Fc fusion protein smearing on SDS PAGE - (reply: 2)
  36. Help with protein expression - how to optimize expression of large GST fusion protein (reply: 4)
  37. effect of lysozyme on gst-fusion protein - (reply: 1)
  38. Problem with Pierce GST spin purification column - (reply: 6)
  39. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
  40. GST-fusion protein degrades in-vivo - (reply: 3)
  41. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
  42. insoluble fusion protein - (reply: 1)
  43. GST-tag refolding? - (reply: 1)
  44. Help with GST protein purification - (reply: 4)
  45. Fusion protein linker - (reply: 2)
  46. Fusion Protein - (reply: 3)
  47. pGEX vectors and GST expression - (reply: 3)
  48. eluting gst fusion proteins - (reply: 1)
  49. GST pulldown: elude or not? - (reply: 1)
  50. gst pulldown - (reply: 2)
  51. Protein elution from GST-fusion protein complex - (reply: 3)
  52. amount of plasmid for transformation and fusion protein expression - (reply: 1)
  53. gst fusion protein - (reply: 3)
  54. Problem with MBP and GST tag purification - (reply: 1)
  55. DTT in GST elution buffer - (reply: 1)
  56. GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
  57. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
  58. purity is so bad after elution from GST column - (reply: 10)
  59. Antibody for Fusion Protein - Antibody/ies against fusion protein (reply: 2)
  60. GST pull down - (reply: 1)
  61. Contamination in GST fusion protein purification - (reply: 2)
  62. problem in the GST fusion protein purification - (reply: 4)
  63. Troubleshooting GST purification - (reply: 4)
  64. Can GST-tag be separated from GST-fusion protein - (reply: 1)
  65. fusion protein construction - (reply: 1)
  66. Problem with Protein elution using GST sepharose 4B - (reply: 1)
  67. Low Thrombin Cleavage Efficiency Problem - I am trying to cleave a GST tagged protein(about 35 kDa + 26 kDa GST) (reply: 1)
  68. Couple questions about GST purification - (reply: 16)
  69. GST-fusion protein purification - How to purify it? (reply: 9)
  70. GST pull down assay - (reply: 3)
  71. GST preotin non specific preotins - Non specific GST bands (reply: 2)
  72. GST-tag protein purification - GST-tag protein purification (reply: 4)
  73. GST fusion protein purification - (reply: 1)
  74. B-per GST fusion protein purification kit - (reply: 2)
  75. A story of Urea, dialysis and GST affinity purification... hopefully! - (reply: 1)
  76. Getting rid of background protein binding on GST beads - (reply: 5)
  77. GST pull down assay - (reply: 4)