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GST-tag Related Discussions
  1. Thrombin cleaved GST-fusion protein- multiple bands - (reply: 2)
  2. Beads pull down only free GST - (reply: 14)
  3. Making a fusion protein - (reply: 1)
  4. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
  5. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
  6. His-tag and GST-tag - (reply: 1)
  7. designing fusion protein with Flag tag - (reply: 1)
  8. Flag fusion protein not detected by Flag antibody - (reply: 8)
  9. Luciferase fusion protein? - (reply: 1)
  10. GST tag fusion protein--- help please - (reply: 4)
  11. Anyone has experience in using estrogen receptor (ER) fusion protein? - (reply: 1)
  12. Difficulty in Expression of Protein Having 73% GC contents in GST Tag Vectrors - (reply: 3)
  13. GST pull down assay: GST binds to the prey protein. Negative control shows bindi - (reply: 4)
  14. Soluble protien of GST - (reply: 2)
  15. Cleavage of GST-tag with Factor Xa - (reply: 1)
  16. Trouble in GST- fusion protein purification - (reply: 1)
  17. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
  18. C-terminal GST fusion problem in mammalian cell - (reply: 6)
  19. minimum recombinant protein amounts for gst pulldown - (reply: 4)
  20. GST TAG - (reply: 3)
  21. Residues on the N terminal GST Tag - (reply: 1)
  22. Peptide recuperation after FactorXa cleavage using GST fusion - (reply: 1)
  23. Why GFP gets cutted from my fusion protein? - (reply: 9)
  24. GST fusion protein purification - (reply: 2)
  25. Could anyone help me out with the removal GST tag from my recombinant protein? - (reply: 1)
  26. GFP fusion protein not expressing! - (reply: 2)
  27. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
  28. GST tag protein- soluble or insoluble??? help! - (reply: 2)
  29. Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
  30. Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
  31. Difficulty in expressing fusion protein - (reply: 1)
  32. what is the molecular weight of fusion protein in pET-32a - molecular weight of - (reply: 1)
  33. Problems eluting a GST fusion protein in GSTrap FF - (reply: 6)
  34. Fc fusion protein smearing on SDS PAGE - (reply: 2)
  35. Help with protein expression - how to optimize expression of large GST fusion protein (reply: 4)
  36. effect of lysozyme on gst-fusion protein - (reply: 1)
  37. Problem with Pierce GST spin purification column - (reply: 6)
  38. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
  39. GST-fusion protein degrades in-vivo - (reply: 3)
  40. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
  41. insoluble fusion protein - (reply: 1)
  42. GST-tag refolding? - (reply: 1)
  43. Help with GST protein purification - (reply: 4)
  44. Fusion protein linker - (reply: 2)
  45. Fusion Protein - (reply: 3)
  46. pGEX vectors and GST expression - (reply: 3)
  47. eluting gst fusion proteins - (reply: 1)
  48. GST pulldown: elude or not? - (reply: 1)
  49. gst pulldown - (reply: 2)
  50. Protein elution from GST-fusion protein complex - (reply: 3)
  51. amount of plasmid for transformation and fusion protein expression - (reply: 1)
  52. gst fusion protein - (reply: 3)
  53. Problem with MBP and GST tag purification - (reply: 1)
  54. DTT in GST elution buffer - (reply: 1)
  55. GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
  56. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
  57. purity is so bad after elution from GST column - (reply: 10)
  58. Antibody for Fusion Protein - Antibody/ies against fusion protein (reply: 2)
  59. GST pull down - (reply: 1)
  60. Contamination in GST fusion protein purification - (reply: 2)
  61. problem in the GST fusion protein purification - (reply: 4)
  62. Troubleshooting GST purification - (reply: 4)
  63. Can GST-tag be separated from GST-fusion protein - (reply: 1)
  64. fusion protein construction - (reply: 1)
  65. Problem with Protein elution using GST sepharose 4B - (reply: 1)
  66. Low Thrombin Cleavage Efficiency Problem - I am trying to cleave a GST tagged protein(about 35 kDa + 26 kDa GST) (reply: 1)
  67. Couple questions about GST purification - (reply: 16)
  68. GST-fusion protein purification - How to purify it? (reply: 9)
  69. GST pull down assay - (reply: 3)
  70. GST preotin non specific preotins - Non specific GST bands (reply: 2)
  71. GST-tag protein purification - GST-tag protein purification (reply: 4)
  72. GST fusion protein purification - (reply: 1)
  73. B-per GST fusion protein purification kit - (reply: 2)
  74. A story of Urea, dialysis and GST affinity purification... hopefully! - (reply: 1)
  75. Getting rid of background protein binding on GST beads - (reply: 5)
  76. GST pull down assay - (reply: 4)