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Flow-Cytometry
31.
PI stain for cell viability~~Compensation? Suggestion needed~~^^ -
(reply: 4)
32.
Propidium iodide color interference -
(reply: 4)
33.
violet laser (405nm) and cell cycle staining with DAPI -
(reply: 1)
34.
Requesting basic protocols on cell sorting -
(reply: 1)
35.
How to draw figures from Flow cytometry data -
(reply: 3)
36.
Problems with fixation? -
(reply: 7)
37.
FACS Buffer -
(reply: 5)
38.
Please help - FACS to ELISA for antibody testing -
(reply: 3)
39.
Suitable fluorophores for low pH -
(reply: 1)
40.
Propidium iodide staining -
(reply: 1)
41.
Analysis of high throughput screen for cell surface markers -
(reply: 1)
42.
IgG control -
(reply: 4)
43.
Annexin binding buffer (urgent advice) -
(reply: 2)
44.
ROS detection using DCFDA -
(reply: 2)
45.
FACS antibody titration -
(reply: 5)
46.
GFP tagged protein expression detection -
(reply: 1)
47.
centrifugation speed/cell prep for flow -
(reply: 4)
48.
ANNEXIN V staining for FLOW -
(reply: 3)
49.
Tween-20 vs Triton-X vs Saponin -
(reply: 1)
50.
What positive control for intracellular staining? -
(reply: 1)
51.
what's that small peak prior to G1? -
(reply: 2)
52.
Fixed cells, sticky cells? -
(reply: 2)
53.
Difference between flow cytometry and immunostaining? -
(reply: 3)
54.
what's more useful in flow cytometry -
(reply: 3)
55.
Help reading/identifying part of a FCM chart. -
(reply: 1)
56.
What if I froze a PE-conjugated antibody -
(reply: 2)
57.
Looking for cell surface markers. -
(reply: 3)
58.
intracellular cytokine staining -
(reply: 1)
59.
staining at 37 degrees or RT - OK or really bad ? -
(reply: 3)
60.
data analysis for single cell size and distribution -
(reply: 1)
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