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Top : New Forum Archives (2009-): : Flow-Cytometry
1. Why GFP/YFP+ neurons couldn't be seen at cytometer after mouse brain dissoci - (reply: 3)
2. Can propidium iodide be used on WBC nuclei with cell membrane removed - (reply: 1)
3. Antibody concentration - (reply: 5)
4. Can I skip the FC blocking step in flow cytometry? - (reply: 3)
5. Ab titration - (reply: 1)
6. FACS with culture media - (reply: 2)
7. How to determine FACS result - (reply: 5)
8. Does collagenase digestion require quenching? - (reply: 2)
9. Basic questions - (reply: 3)
10. Optimization of the flow cytometry - (reply: 3)
11. cell viability with PI staining - (reply: 2)
12. Cell Cycle Analysis - crucial to keep cells cold after ethanol fixation, and aft - (reply: 1)
13. Made a mistake and fixed cells with 30% ethanol for cell cycle analysis. Can I s - (reply: 1)
14. fixation and permeabilization - (reply: 2)
15. Quantuitative staining of RNA and DNA? - (reply: 4)
16. Viability dye 7-AAD - (reply: 3)
17. Cell cycle determination - (reply: 1)
18. Newbie at Flow cytometry so I need a lot of help - (reply: 7)
19. Biotin-streptavidin signal amplification - (reply: 2)
20. Excitation of fluorphores using UV for microscope vs lasers for cytometry - (reply: 3)
21. Mean of fluorescence in FlowJo - (reply: 1)
22. PI staining cell cycle analysis - (reply: 1)
23. PI, CdCl2 and cytotoxicity - (reply: 4)
24. FACS Canto II not showing events - (reply: 3)
25. PBMC FACS - (reply: 2)
26. What should I suspend my cells in prior to cytometry? - (reply: 2)
27. Protocol for flow cytometry on mononuclear cells from mouse blood - (reply: 1)
28. Please help... - (reply: 2)
29. Annexin V clone - (reply: 2)
30. preparation of cells for FACS analysis after transfection - (reply: 4)

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