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Top : New Forum Archives (2009-): : Flow-Cytometry
1. Counting beads binding to RBCs - (reply: 1)
2. What are the uses for positive and negative controls in flow cytometry? - (reply: 4)
3. Why GFP/YFP+ neurons couldn't be seen at cytometer after mouse brain dissoci - (reply: 3)
4. Can propidium iodide be used on WBC nuclei with cell membrane removed - (reply: 1)
5. Antibody concentration - (reply: 5)
6. Can I skip the FC blocking step in flow cytometry? - (reply: 3)
7. Ab titration - (reply: 1)
8. FACS with culture media - (reply: 2)
9. How to determine FACS result - (reply: 5)
10. Does collagenase digestion require quenching? - (reply: 2)
11. Basic questions - (reply: 3)
12. Optimization of the flow cytometry - (reply: 3)
13. cell viability with PI staining - (reply: 2)
14. Cell Cycle Analysis - crucial to keep cells cold after ethanol fixation, and aft - (reply: 1)
15. Made a mistake and fixed cells with 30% ethanol for cell cycle analysis. Can I s - (reply: 1)
16. fixation and permeabilization - (reply: 2)
17. Quantuitative staining of RNA and DNA? - (reply: 4)
18. Viability dye 7-AAD - (reply: 4)
19. Cell cycle determination - (reply: 1)
20. Newbie at Flow cytometry so I need a lot of help - (reply: 7)
21. Biotin-streptavidin signal amplification - (reply: 2)
22. Excitation of fluorphores using UV for microscope vs lasers for cytometry - (reply: 3)
23. Mean of fluorescence in FlowJo - (reply: 1)
24. PI staining cell cycle analysis - (reply: 1)
25. PI, CdCl2 and cytotoxicity - (reply: 4)
26. FACS Canto II not showing events - (reply: 3)
27. PBMC FACS - (reply: 2)
28. What should I suspend my cells in prior to cytometry? - (reply: 2)
29. Protocol for flow cytometry on mononuclear cells from mouse blood - (reply: 1)
30. Please help... - (reply: 2)

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