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1. Two distinct populations observed on a homogenous cells - (reply: 1)
2. Fixation for surface epitope detection - (reply: 1)
3. negative control readings - (reply: 1)
4. No-wash fixation/permeabilization kit for RBC - (reply: 1)
5. Help with the Red Blood Cell Lysis - (reply: 1)
6. Counting beads binding to RBCs - (reply: 1)
7. What are the uses for positive and negative controls in flow cytometry? - (reply: 6)
8. Annexin PI staining - (reply: 1)
9. Why GFP/YFP+ neurons couldn't be seen at cytometer after mouse brain dissoci - (reply: 3)
10. Can propidium iodide be used on WBC nuclei with cell membrane removed - (reply: 1)
11. How to clean up cell fragments before cell sorting - (reply: 1)
12. Antibody concentration - (reply: 5)
13. Can I skip the FC blocking step in flow cytometry? - (reply: 3)
14. Ab titration - (reply: 1)
15. FACS with culture media - (reply: 2)
16. How to determine FACS result - (reply: 5)
17. Does collagenase digestion require quenching? - (reply: 2)
18. Basic questions - (reply: 3)
19. Optimization of the flow cytometry - (reply: 3)
20. cell viability with PI staining - (reply: 2)
21. Cell Cycle Analysis - crucial to keep cells cold after ethanol fixation, and aft - (reply: 1)
22. Made a mistake and fixed cells with 30% ethanol for cell cycle analysis. Can I s - (reply: 1)
23. fixation and permeabilization - (reply: 2)
24. Quantuitative staining of RNA and DNA? - (reply: 4)
25. Viability dye 7-AAD - (reply: 4)
26. Cell cycle determination - (reply: 1)
27. Newbie at Flow cytometry so I need a lot of help - (reply: 7)
28. Biotin-streptavidin signal amplification - (reply: 2)
29. Excitation of fluorphores using UV for microscope vs lasers for cytometry - (reply: 3)
30. Mean of fluorescence in FlowJo - (reply: 1)
2. Fixation for surface epitope detection - (reply: 1)
3. negative control readings - (reply: 1)
4. No-wash fixation/permeabilization kit for RBC - (reply: 1)
5. Help with the Red Blood Cell Lysis - (reply: 1)
6. Counting beads binding to RBCs - (reply: 1)
7. What are the uses for positive and negative controls in flow cytometry? - (reply: 6)
8. Annexin PI staining - (reply: 1)
9. Why GFP/YFP+ neurons couldn't be seen at cytometer after mouse brain dissoci - (reply: 3)
10. Can propidium iodide be used on WBC nuclei with cell membrane removed - (reply: 1)
11. How to clean up cell fragments before cell sorting - (reply: 1)
12. Antibody concentration - (reply: 5)
13. Can I skip the FC blocking step in flow cytometry? - (reply: 3)
14. Ab titration - (reply: 1)
15. FACS with culture media - (reply: 2)
16. How to determine FACS result - (reply: 5)
17. Does collagenase digestion require quenching? - (reply: 2)
18. Basic questions - (reply: 3)
19. Optimization of the flow cytometry - (reply: 3)
20. cell viability with PI staining - (reply: 2)
21. Cell Cycle Analysis - crucial to keep cells cold after ethanol fixation, and aft - (reply: 1)
22. Made a mistake and fixed cells with 30% ethanol for cell cycle analysis. Can I s - (reply: 1)
23. fixation and permeabilization - (reply: 2)
24. Quantuitative staining of RNA and DNA? - (reply: 4)
25. Viability dye 7-AAD - (reply: 4)
26. Cell cycle determination - (reply: 1)
27. Newbie at Flow cytometry so I need a lot of help - (reply: 7)
28. Biotin-streptavidin signal amplification - (reply: 2)
29. Excitation of fluorphores using UV for microscope vs lasers for cytometry - (reply: 3)
30. Mean of fluorescence in FlowJo - (reply: 1)