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Flow-Cytometry
121.
Facs for BrdU and other staining - double intranuclear + GFP
(reply: 2)
122.
Gating of annexin V PI assay -
(reply: 7)
123.
problem with cell cycle analysis -
(reply: 3)
124.
How to choose a flow cytometer -
(reply: 1)
125.
Ethidium bromide protocol -
(reply: 2)
126.
FACS - double staining - apoptosis AND proliferation markers
(reply: 4)
127.
cell sorting - losing cells? -
(reply: 3)
128.
Saving tissue and single cell suspension - FACS, single cell suspension
(reply: 1)
129.
Two peaks measured by DCFH-DA -
(reply: 1)
130.
histogram overlay - how to do this in FACSDiva
(reply: 1)
131.
Annexin V assay analysis -
(reply: 10)
132.
Flow Cytometry using Accuri systems - advise on the analysis using this program?
(reply: 1)
133.
Propodium Iodide postive and Annexin V negative -
(reply: 1)
134.
How to determine Kd -
(reply: 1)
135.
negative control -
(reply: 3)
136.
RBC Lysis and Apoptosis - does it interfere?
(reply: 2)
137.
Surface Staining and Methanol Fixation -
(reply: 1)
138.
Live stain with propidium iodide - Incubation time & temperature?
(reply: 3)
139.
DCF Assay to detect oxidative stress - Fluorescence lower than expected - recommended improvements?
(reply: 8)
140.
Rnase for flow cytometry -
(reply: 1)
141.
Detect apoptosis by FACS -
(reply: 1)
142.
How to culture cells after sorting? -
(reply: 3)
143.
Good antibody for E. coli surface marker - phagocytosis assay
(reply: 2)
144.
Flow cytometry of RFP and CFP expressing yeast cells -
(reply: 1)
145.
Quantifying histogram data - Flow Cytometry analysis
(reply: 2)
146.
Do my antibodies need to stay sterile? -
(reply: 5)
147.
RBC Lysis of Rodent Peripheral Blood - which protocol?
(reply: 1)
148.
Expired conjugated Antibodies? -
(reply: 4)
149.
FBS Annexin staining -
(reply: 1)
150.
ROS detection with dihydroethidium -
(reply: 3)
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