Can I skip the FC blocking step in flow cytometry? - (Feb/03/2015 )
Can I skip the FC blocking step in flow cytometry? and when I shouldn't do that?
-H. Aly-
You can skip it but it might lead to a higher background. I wouldn't do it when staining macrophages or monocytes since they have a lot of Fc receptors
-Tabaluga-
As Tab said, however, you can do away this step if you are staining whole blood (because there is a lot of serum in the whole blood blocking the Fc receptors).
-CPRES-
what about staining a monolayer cell line not blood ?
-H. Aly-