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Top : New Forum Archives (2009-): : Flow-Cytometry
1. No-wash fixation/permeabilization kit for RBC - (reply: 1)
2. Help with the Red Blood Cell Lysis - (reply: 1)
3. Counting beads binding to RBCs - (reply: 1)
4. What are the uses for positive and negative controls in flow cytometry? - (reply: 6)
5. Annexin PI staining - (reply: 1)
6. Why GFP/YFP+ neurons couldn't be seen at cytometer after mouse brain dissoci - (reply: 3)
7. Can propidium iodide be used on WBC nuclei with cell membrane removed - (reply: 1)
8. How to clean up cell fragments before cell sorting - (reply: 1)
9. Antibody concentration - (reply: 5)
10. Can I skip the FC blocking step in flow cytometry? - (reply: 3)
11. Ab titration - (reply: 1)
12. FACS with culture media - (reply: 2)
13. How to determine FACS result - (reply: 5)
14. Does collagenase digestion require quenching? - (reply: 2)
15. Basic questions - (reply: 3)
16. Optimization of the flow cytometry - (reply: 3)
17. cell viability with PI staining - (reply: 2)
18. Cell Cycle Analysis - crucial to keep cells cold after ethanol fixation, and aft - (reply: 1)
19. Made a mistake and fixed cells with 30% ethanol for cell cycle analysis. Can I s - (reply: 1)
20. fixation and permeabilization - (reply: 2)
21. Quantuitative staining of RNA and DNA? - (reply: 4)
22. Viability dye 7-AAD - (reply: 4)
23. Cell cycle determination - (reply: 1)
24. Newbie at Flow cytometry so I need a lot of help - (reply: 7)
25. Biotin-streptavidin signal amplification - (reply: 2)
26. Excitation of fluorphores using UV for microscope vs lasers for cytometry - (reply: 3)
27. Mean of fluorescence in FlowJo - (reply: 1)
28. PI staining cell cycle analysis - (reply: 1)
29. PI, CdCl2 and cytotoxicity - (reply: 4)
30. FACS Canto II not showing events - (reply: 3)

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