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1. FACS staining of human macrophages derived from PBMCs - (reply: 2)
2. PI stain for cell viability~~Compensation? Suggestion needed~~^^ - (reply: 2)
3. Propidium iodide color interference - (reply: 3)
4. violet laser (405nm) and cell cycle staining with DAPI - (reply: 1)
5. Requesting basic protocols on cell sorting - (reply: 1)
6. How to draw figures from Flow cytometry data - (reply: 3)
7. Problems with fixation? - (reply: 7)
8. FACS Buffer - (reply: 5)
9. Please help - FACS to ELISA for antibody testing - (reply: 3)
10. Suitable fluorophores for low pH - (reply: 1)
11. Propidium iodide staining - (reply: 1)
12. Analysis of high throughput screen for cell surface markers - (reply: 1)
13. IgG control - (reply: 4)
14. Annexin binding buffer (urgent advice) - (reply: 2)
15. ROS detection using DCFDA - (reply: 2)
16. FACS antibody titration - (reply: 5)
17. GFP tagged protein expression detection - (reply: 1)
18. centrifugation speed/cell prep for flow - (reply: 2)
19. ANNEXIN V staining for FLOW - (reply: 3)
20. Tween-20 vs Triton-X vs Saponin - (reply: 1)
21. What positive control for intracellular staining? - (reply: 1)
22. what's that small peak prior to G1? - (reply: 2)
23. Fixed cells, sticky cells? - (reply: 2)
24. Difference between flow cytometry and immunostaining? - (reply: 3)
25. what's more useful in flow cytometry - (reply: 3)
26. Help reading/identifying part of a FCM chart. - (reply: 1)
27. What if I froze a PE-conjugated antibody - (reply: 2)
28. Looking for cell surface markers. - (reply: 3)
29. intracellular cytokine staining - (reply: 1)
30. staining at 37 degrees or RT - OK or really bad ? - (reply: 3)
2. PI stain for cell viability~~Compensation? Suggestion needed~~^^ - (reply: 2)
3. Propidium iodide color interference - (reply: 3)
4. violet laser (405nm) and cell cycle staining with DAPI - (reply: 1)
5. Requesting basic protocols on cell sorting - (reply: 1)
6. How to draw figures from Flow cytometry data - (reply: 3)
7. Problems with fixation? - (reply: 7)
8. FACS Buffer - (reply: 5)
9. Please help - FACS to ELISA for antibody testing - (reply: 3)
10. Suitable fluorophores for low pH - (reply: 1)
11. Propidium iodide staining - (reply: 1)
12. Analysis of high throughput screen for cell surface markers - (reply: 1)
13. IgG control - (reply: 4)
14. Annexin binding buffer (urgent advice) - (reply: 2)
15. ROS detection using DCFDA - (reply: 2)
16. FACS antibody titration - (reply: 5)
17. GFP tagged protein expression detection - (reply: 1)
18. centrifugation speed/cell prep for flow - (reply: 2)
19. ANNEXIN V staining for FLOW - (reply: 3)
20. Tween-20 vs Triton-X vs Saponin - (reply: 1)
21. What positive control for intracellular staining? - (reply: 1)
22. what's that small peak prior to G1? - (reply: 2)
23. Fixed cells, sticky cells? - (reply: 2)
24. Difference between flow cytometry and immunostaining? - (reply: 3)
25. what's more useful in flow cytometry - (reply: 3)
26. Help reading/identifying part of a FCM chart. - (reply: 1)
27. What if I froze a PE-conjugated antibody - (reply: 2)
28. Looking for cell surface markers. - (reply: 3)
29. intracellular cytokine staining - (reply: 1)
30. staining at 37 degrees or RT - OK or really bad ? - (reply: 3)