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Top : New Forum Archives (2009-): : Flow-Cytometry
1. Fixation for surface epitope detection - (reply: 1)
2. negative control readings - (reply: 1)
3. No-wash fixation/permeabilization kit for RBC - (reply: 1)
4. Help with the Red Blood Cell Lysis - (reply: 1)
5. Counting beads binding to RBCs - (reply: 1)
6. What are the uses for positive and negative controls in flow cytometry? - (reply: 6)
7. Annexin PI staining - (reply: 1)
8. Why GFP/YFP+ neurons couldn't be seen at cytometer after mouse brain dissoci - (reply: 3)
9. Can propidium iodide be used on WBC nuclei with cell membrane removed - (reply: 1)
10. How to clean up cell fragments before cell sorting - (reply: 1)
11. Antibody concentration - (reply: 5)
12. Can I skip the FC blocking step in flow cytometry? - (reply: 3)
13. Ab titration - (reply: 1)
14. FACS with culture media - (reply: 2)
15. How to determine FACS result - (reply: 5)
16. Does collagenase digestion require quenching? - (reply: 2)
17. Basic questions - (reply: 3)
18. Optimization of the flow cytometry - (reply: 3)
19. cell viability with PI staining - (reply: 2)
20. Cell Cycle Analysis - crucial to keep cells cold after ethanol fixation, and aft - (reply: 1)
21. Made a mistake and fixed cells with 30% ethanol for cell cycle analysis. Can I s - (reply: 1)
22. fixation and permeabilization - (reply: 2)
23. Quantuitative staining of RNA and DNA? - (reply: 4)
24. Viability dye 7-AAD - (reply: 4)
25. Cell cycle determination - (reply: 1)
26. Newbie at Flow cytometry so I need a lot of help - (reply: 7)
27. Biotin-streptavidin signal amplification - (reply: 2)
28. Excitation of fluorphores using UV for microscope vs lasers for cytometry - (reply: 3)
29. Mean of fluorescence in FlowJo - (reply: 1)
30. PI staining cell cycle analysis - (reply: 1)

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