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Top : New Forum Archives (2009-): : Flow-Cytometry
1. negative control readings - (reply: 1)
2. No-wash fixation/permeabilization kit for RBC - (reply: 1)
3. Help with the Red Blood Cell Lysis - (reply: 1)
4. Counting beads binding to RBCs - (reply: 1)
5. What are the uses for positive and negative controls in flow cytometry? - (reply: 6)
6. Annexin PI staining - (reply: 1)
7. Why GFP/YFP+ neurons couldn't be seen at cytometer after mouse brain dissoci - (reply: 3)
8. Can propidium iodide be used on WBC nuclei with cell membrane removed - (reply: 1)
9. How to clean up cell fragments before cell sorting - (reply: 1)
10. Antibody concentration - (reply: 5)
11. Can I skip the FC blocking step in flow cytometry? - (reply: 3)
12. Ab titration - (reply: 1)
13. FACS with culture media - (reply: 2)
14. How to determine FACS result - (reply: 5)
15. Does collagenase digestion require quenching? - (reply: 2)
16. Basic questions - (reply: 3)
17. Optimization of the flow cytometry - (reply: 3)
18. cell viability with PI staining - (reply: 2)
19. Cell Cycle Analysis - crucial to keep cells cold after ethanol fixation, and aft - (reply: 1)
20. Made a mistake and fixed cells with 30% ethanol for cell cycle analysis. Can I s - (reply: 1)
21. fixation and permeabilization - (reply: 2)
22. Quantuitative staining of RNA and DNA? - (reply: 4)
23. Viability dye 7-AAD - (reply: 4)
24. Cell cycle determination - (reply: 1)
25. Newbie at Flow cytometry so I need a lot of help - (reply: 7)
26. Biotin-streptavidin signal amplification - (reply: 2)
27. Excitation of fluorphores using UV for microscope vs lasers for cytometry - (reply: 3)
28. Mean of fluorescence in FlowJo - (reply: 1)
29. PI staining cell cycle analysis - (reply: 1)
30. PI, CdCl2 and cytotoxicity - (reply: 4)

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