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Top : New Forum Archives (2009-): : Flow-Cytometry
1. Counting beads binding to RBCs - (reply: 1)
2. What are the uses for positive and negative controls in flow cytometry? - (reply: 4)
3. Annexin PI staining - (reply: 1)
4. Why GFP/YFP+ neurons couldn't be seen at cytometer after mouse brain dissoci - (reply: 3)
5. Can propidium iodide be used on WBC nuclei with cell membrane removed - (reply: 1)
6. How to clean up cell fragments before cell sorting - (reply: 1)
7. Antibody concentration - (reply: 5)
8. Can I skip the FC blocking step in flow cytometry? - (reply: 3)
9. Ab titration - (reply: 1)
10. FACS with culture media - (reply: 2)
11. How to determine FACS result - (reply: 5)
12. Does collagenase digestion require quenching? - (reply: 2)
13. Basic questions - (reply: 3)
14. Optimization of the flow cytometry - (reply: 3)
15. cell viability with PI staining - (reply: 2)
16. Cell Cycle Analysis - crucial to keep cells cold after ethanol fixation, and aft - (reply: 1)
17. Made a mistake and fixed cells with 30% ethanol for cell cycle analysis. Can I s - (reply: 1)
18. fixation and permeabilization - (reply: 2)
19. Quantuitative staining of RNA and DNA? - (reply: 4)
20. Viability dye 7-AAD - (reply: 4)
21. Cell cycle determination - (reply: 1)
22. Newbie at Flow cytometry so I need a lot of help - (reply: 7)
23. Biotin-streptavidin signal amplification - (reply: 2)
24. Excitation of fluorphores using UV for microscope vs lasers for cytometry - (reply: 3)
25. Mean of fluorescence in FlowJo - (reply: 1)
26. PI staining cell cycle analysis - (reply: 1)
27. PI, CdCl2 and cytotoxicity - (reply: 4)
28. FACS Canto II not showing events - (reply: 3)
29. PBMC FACS - (reply: 2)
30. What should I suspend my cells in prior to cytometry? - (reply: 2)

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