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Protocols
- DNA Preparation from Adherent Cells
(Thomas Ried's Lab, NCI)
Standard protocol for DNA extraction from cultured adherent cells
http://www.riedlab.nci.nih.gov/publications/2437.2...
Added: Fri Oct 18 2002, Hits: 23438, Reviews: 0 Write review - DNA Preparation from Fresh/Frozen Tissue (Thomas Ried's Lab, NCI)
Standard method for DNA preparation from tissue involving organic extraction.
http://www.riedlab.nci.nih.gov/publications/2439.2...
Added: Fri Oct 18 2002, Hits: 20211, Reviews: 0 Write review -
DNA Extraction from Ancient Tissue
There are two major questions regarding getting DNA out of museum specimens: 1) what's the best tissue to sample, and 2) what's the best way to get DNA out of that tissue? This protocol describes various methods in great detail.
http://www.uga.edu/srel/DNA_Lab/OldDNA99.rtf
Added: Mon Sep 23 2002, Hits: 3380, Reviews: 0 Write review Cached -
DNA Isolation from Buccal Cell
(Daniel T. O'Connor, UCSD Chromaffin Cell and Hypertension Research )
Simple method for obtaining DNA from buccal cells for PCR.
http://hypertension.ucsd.edu/list_of_protocols/31_...
Added: Tue Feb 17 2004, Hits: 5851, Reviews: 0 Write review Cached -
DNA Isolation from Tissues via Cryostections
(Institute of Pathology,Humboldt-University of Berlin)
For extraction of tumore DNA
http://allserv.rug.ac.be/~fspelema/neubla/protocol...
Added: Mon Nov 18 2002, Hits: 2415, Reviews: 0 Write review Cached -
DNA Preparation from Cell Lines
(Thomas Ried's Lab, NCI)
High salt method of DNA extraction without organic extraction.
http://www.riedlab.nci.nih.gov/publications/2442.2...
Added: Fri Oct 18 2002, Hits: 15725, Reviews: 0 Write review -
ES Cell DNA Extraction: Tube Method
(Hedrick Lab, UCSD Cancer Center)
Protocol for extracting DNA from ES Cells, starting from the 96-well plate but processing in an eppendorf tube to recover more of the DNA
http://hedricklab.ucsd.edu/Protocol/ESCell.html
Added: Tue Dec 10 2002, Hits: 3372, Reviews: 0 Write review Cached -
Genomic DNA Quickprep for PCR
(Kay Schneitz Lab)
Very simple method of preparing DNA for PCR with recipes
http://www.unizh.ch/botinst/Cyto_Website/schneitzL...
Added: Tue May 14 2002, Hits: 8159, Reviews: 0 Write review Cached -
Isolation of Mouse Genomic DNA
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
Mouse liver DNA extraction
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 618, Reviews: 0 Write review Cached -
Mouse and Human Genomic DNA Extraction
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
Extract DNA from frozen blood
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 644, Reviews: 0 Write review Cached -
Nucleic Acid Isolation and Purification Manual
(BMB)
This 180-page manual collects a wide variety of reliable protocols and technical tips and will enable readers to quickly find the most optimal method for their specific needs.
http://206.53.227.20/prod_inf/manuals/napi_man/nap...
Added: Tue May 14 2002, Hits: 884, Reviews: 0 Write review Cached -
Phenol extraction of DNA
(Roe Lab)
http://www.genome.ou.edu/protocol_book/protocol_pa...
Added: Tue May 14 2002, Hits: 7755, Reviews: 0 Write review Cached -
Phenol/Chloroform Extraction of DNA
(Julie B. Wolf, University of Maryland, Baltimore County)
http://www.research.umbc.edu/~jwolf/m4.htm#phenol
Added: Tue May 14 2002, Hits: 10494, Reviews: 0 Write review Cached - Rapid Isolation of Genomic DNA from Human Oral Mucosa
This protocol describes a quick method to purify genomic DNA from human oral mucosa.
Added: Mon Feb 02 2009, Reviews: 0 Write review -
Salting Out Procedure for Human DNA Extraction
(Donis Keller Lab)
This procedure avoids using phenol and chloroform by using high salt concentrations to remove proteins. It is rapid, safe and inexpensive.
http://hg.wustl.edu/hdk_lab_manual/dna/dna2.html
Added: Tue May 14 2002, Hits: 3049, Reviews: 0 Write review Cached - Simplified DNA Extraction from Cell or Tissue (Protocol Online)
This method doesn't require organic extraction and centrifugation. It's the most simplest way of preparing DNA and works great.
Added: Tue May 14 2002, Reviews: 1 Read reviews Write review