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Genomic DNA Quickprep for PCR

Zürich Online Bibliothek Uni ZH Vorlesungen Institute of Plant Biology Home Page U of Zürich Home Page   Schneitz Lab Home Page / Methods / DNA Work / Genomic DNA Quickprep for PCR
Genomic DNA Quickprep for PCR
Reference:  Edwards, 1991, NAR 19: 1349 Last updated: 12/5/99 By: Kay Schneitz
  1. macerate tissue in Eppendorf tube without butter at RT
  2. add 400 ml extraction buffer
  3. vortex for 4 sec
  4. leave sample at RT until other samples are ready (> 1 h)
  5. spin in microfuge for 1 min
  6. transfer 300 ml of supernatant to different Eppendorf tube (prefilled with 300 ml isopropanole)
  7. mix and leave at RT for 2 min
  8. spin for 5 min
  9. vacuum dry pellet and take up in 100 ml TE
  10. use 1-2.5 ml for PCR



DNA is stable for one year at 4°C




Extraction buffer:

200 mM Tris-HCl pH 7.5
250 mM NaCl
 25 mM EDTA
0.5%  SDS


Reagent/Tool Supplier Cat.-#
Piston Pellet Treff Polylabo SA, Geneva 82855

  © Copyright 1997-1999, Kay Schneitz. Last updated 1/17/99; 5:47:25 PM. Send comments to the webmeister.