| Genomic DNA Quickprep for PCR | ||
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| Reference: Edwards et.al., 1991, NAR 19: 1349 | Last updated: 12/5/99 | By: Kay Schneitz |
- macerate tissue in Eppendorf tube without butter at RT
- add 400 ml extraction buffer
- vortex for 4 sec
- leave sample at RT until other samples are ready (> 1 h)
- spin in microfuge for 1 min
- transfer 300 ml of supernatant to different Eppendorf tube (prefilled with 300 ml isopropanole)
- mix and leave at RT for 2 min
- spin for 5 min
- vacuum dry pellet and take up in 100 ml TE
- use 1-2.5 ml for PCR
Remarks:
DNA is stable for one year at 4°C
Solutions: | ||
Extraction buffer: 200 mM Tris-HCl pH 7.5250 mM NaCl 25 mM EDTA 0.5% SDS | ||
Materials:
| Reagent/Tool | Supplier | Cat.-# |
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| Piston Pellet Treff | Polylabo SA, Geneva | 82855 |