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CIP - (Dec/05/2005 )

I need a verification

when do I use CIP (alkaline phosphatase) after cuting with ristrection enzyme???

do I use it always or just when cutting with blunt ends



use CIP on your vector after RE digest if re-ligation of the vector to self is a possibility

never use CIP on your insert, and if you have used two enzymes and your ends are incompatible it should not be necessary

does this help?


THANKS biggrin.gif


Use shrimp alkaline phosphatase or antarctic phosphatase rather than CIP. Both are much less likely to cause other problems with your DNA, and can be heat inactivated.
Use the correct number of units -- an excess of enzyme can cause problems here.