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phosphatase inhibitors - what do I use for with ant-P-Ser IP? (Jul/26/2004 )

I want to do phospho-IP from mammalian cell extracts and am trying to select phospahtase inhibitors while avoiding anything too toxic (if possible).

Any have any ideas or experience with this?



You can use Caliculin A for Serine/theronine phosphatases and sodium vanadate for Tyrosine phosphostases.

Just add these to your sample buffer when lysing your cells.