phosphatase inhibitors - what do I use for with ant-P-Ser IP? (Jul/26/2004 )
I want to do phospho-IP from mammalian cell extracts and am trying to select phospahtase inhibitors while avoiding anything too toxic (if possible).
Any have any ideas or experience with this?
You can use Caliculin A for Serine/theronine phosphatases and sodium vanadate for Tyrosine phosphostases.
Just add these to your sample buffer when lysing your cells.