His tagged Flagellin purification - protein going into inclusion bodies (Jul/31/2003 )
I am trying to purify cloned His tagged Flagellin from E.Coli host using Ni-NTA agarose spin column (Quiagen). For my work I want the protein in Native form, but after induction the protein is ending up in inclusion bodies and I can't solubilize it. I have tried inducing it with less IPTG but even that doesn't seem to work.
Pl. suggest if there is a better way to do it.
I mean to purify it in its native form as if I use the denaturing conditions I am afraid that it won't fold back in its original form.
try growing your culture and inducing at 28 degrees C.