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Protocols
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Butanol Extraction of Oligonucleotides
(Synthegen)
Protocol for Desalting Oligonucleotides Using Butanol Extraction
http://www.synthegen.com/technical/protocols/butan...
Added: Sun Oct 26 2003, Hits: 1454, Reviews: 0 Write review Cached -
Butanol Precipitation of Deprotected Oligonucleotides
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
Crude oligonucleotides can be precipitated after deprotection directly from the ammonia solution without the need for a separate step to remove the ammonia. This procedure removes small molecular weight impurities such as the ammonia itself and the by products of deprotection (benzamide, isobutyramide).
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 107, Reviews: 0 Write review Cached -
Desalting Oligonucleotides by Ethanol Precipitation
(Synthegen)
Protocol for desalting oligonucleotides using ethanol precipitation
http://www.synthegen.com/technical/protocols/ethan...
Added: Sun Oct 26 2003, Hits: 2068, Reviews: 0 Write review Cached -
Desalting Oligonucleotides Using Gel Filtration
(Synthegen)
General procedures for desalting oligonucleotides by gel filtration
http://www.synthegen.com/technical/protocols/gel_f...
Added: Sun Oct 26 2003, Hits: 1025, Reviews: 0 Write review Cached -
Isolation of DNA from Acrylamide Gels
(Schimenti Lab)
http://lena.jax.org/~jcs/techniques/protocols/acry...
Added: Tue May 14 2002, Hits: 126, Reviews: 0 Write review Cached -
Oligo Concentration by Butanol Extraction
(John Roth, Division of Biological Sciences,University of California at Davis)
http://rothlab.ucdavis.edu/protocols/P30.html
Added: Tue May 25 2004, Hits: 830, Reviews: 0 Write review Cached -
Oligo Purification on Acrylamide Gels
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 145, Reviews: 0 Write review Cached -
Oligonucleotide Purification
(IDT)
http://www.idtdna.com/program/techbulletins/Oligon...
Added: Tue May 14 2002, Hits: 194, Reviews: 0 Write review Cached -
Oligonucleotide purification
(Frank Bottone Jr.)
Buanol precipitation method
http://www.unc.edu/~fbottone/protocol/oligo.html
Added: Tue May 14 2002, Hits: 1234, Reviews: 0 Write review Cached -
Oligonucleotide Purification to Remove Trityl Group
(John Roth, Division of Biological Sciences,University of California at Davis)
To remove trityl group from newly-synthesized oligonucleotide
http://rothlab.ucdavis.edu/protocols/P21.html
Added: Tue May 25 2004, Hits: 664, Reviews: 0 Write review Cached -
Polyacrylamide Gel Electrophoresis of Oligonucleotides
(John Roth, Division of Biological Sciences,University of California at Davis)
http://rothlab.ucdavis.edu/protocols/E11.html
Added: Sun Jun 06 2004, Hits: 4033, Reviews: 0 Write review Cached -
Removal of 32P-ATP from Oligonucleoides
(John Roth, Division of Biological Sciences,University of California at Davis)
Column purification method
http://rothlab.ucdavis.edu/protocols/P20.html
Added: Tue May 25 2004, Hits: 601, Reviews: 0 Write review Cached