Subcategories

Protocols

• Chemistry of Oligonucleotide Synthesis (IDT)
  Introducing basic theory and methods of oligo synthesis.
  http://www.idtdna.com/program/techbulletins/chemis...
  Added: Tue May 14 2002, Hits: 677, Reviews: 0  Write review   Cached
• Chemistry of Oligonucleotide Synthesis (Thermo Hybaid)
  Methods for olgio synthesis, purification, quantitation and labeling.
  http://invitrogen.custhelp.com/cgi-bin/invitrogen....
  Added: Tue May 14 2002, Hits: 879, Reviews: 0  Write review   Cached
• Handling, Dilution and Storage of Oligonucleotides (Pat Heslop-Harrison, Molecular Cytogenetics, University of Leicester)
  http://www.le.ac.uk/biology/phh4/oligos.htm
  Added: Fri May 13 2005, Hits: 752, Reviews: 0  Write review   Cached
• Reconstituting Primers (OpenWetWare)
  Describes procedures for resuspending dry oligonucleotide primers from a manufacturer in buffer. The proper choice of buffer will depend on the intended application of the primers
  http://openwetware.org/wiki/Reconstituting_primers
  Added: Sun Mar 04 2007, Hits: 393, Reviews: 0  Write review   Cached
• Resuspending Lyophilized Oligos (IDT)
  http://www.idtdna.com/program/techbulletins/Resusp...
  Added: Tue May 14 2002, Hits: 301, Reviews: 0  Write review   Cached
• Storage and Handling of Oligos (Sigma)
  Proper storage of your oligonucleotide will maximize its shelf-life, allowing you to get the most use from your oligo. Following these simple dilution and weight/volume unit conversion guidelines will enable you to work easily with your oligos.
  http://www.sigmaaldrich.com/Brands/Sigma_Genosys/C...
  Added: Tue May 14 2002, Hits: 871, Reviews: 0  Write review   Cached

Trouble Shootings and FAQs

• reconstitute and store oligos
• How to prepare double-stranded oligonucleotides from synthesized oligos?
• Cloning using double-stranded oligos - Tips on preparation of oligos and vector, prevention of concatamer formation
• How to clone annealed oligos into a vector
• Removing Oligonucleotides from other DNA