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• DNA Purification

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Protocols

• Butanol Extraction of Oligonucleotides (Synthegen)
  Protocol for Desalting Oligonucleotides Using Butanol Extraction
  http://www.synthegen.com/technical/protocols/butan...
  Added: Sun Oct 26 2003, Hits: 821, Reviews: 0  Write review   Cached
• Butanol Precipitation of Deprotected Oligonucleotides (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  Crude oligonucleotides can be precipitated after deprotection directly from the ammonia solution without the need for a separate step to remove the ammonia. This procedure removes small molecular weight impurities such as the ammonia itself and the by products of deprotection (benzamide, isobutyramide).
  http://www.pmci.unimelb.edu.au/core_facilities/man...
  Added: Tue May 14 2002, Hits: 107, Reviews: 0  Write review   Cached
• Desalting Oligonucleotides by Ethanol Precipitation (Synthegen)
  Protocol for desalting oligonucleotides using ethanol precipitation
  http://www.synthegen.com/technical/protocols/ethan...
  Added: Sun Oct 26 2003, Hits: 1224, Reviews: 0  Write review   Cached
• Desalting Oligonucleotides Using Gel Filtration (Synthegen)
  General procedures for desalting oligonucleotides by gel filtration
  http://www.synthegen.com/technical/protocols/gel_f...
  Added: Sun Oct 26 2003, Hits: 551, Reviews: 0  Write review   Cached
• Isolation of DNA from Acrylamide Gels (Schimenti Lab)
  http://lena.jax.org/~jcs/techniques/protocols/acry...
  Added: Tue May 14 2002, Hits: 126, Reviews: 0  Write review   Cached
• Oligo Concentration by Butanol Extraction (John Roth, Division of Biological Sciences,University of California at Davis)
  http://rothlab.ucdavis.edu/protocols/P30.html
  Added: Tue May 25 2004, Hits: 391, Reviews: 0  Write review   Cached
• Oligo Purification on Acrylamide Gels (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  http://www.pmci.unimelb.edu.au/core_facilities/man...
  Added: Tue May 14 2002, Hits: 145, Reviews: 0  Write review   Cached
• Oligonucleotide Purification (IDT)
  http://www.idtdna.com/program/techbulletins/Oligon...
  Added: Tue May 14 2002, Hits: 194, Reviews: 0  Write review   Cached
• Oligonucleotide purification (Frank Bottone Jr.)
  Buanol precipitation method
  http://www.unc.edu/~fbottone/protocol/oligo.html
  Added: Tue May 14 2002, Hits: 727, Reviews: 0  Write review   Cached
• Oligonucleotide Purification to Remove Trityl Group (John Roth, Division of Biological Sciences,University of California at Davis)
  To remove trityl group from newly-synthesized oligonucleotide
  http://rothlab.ucdavis.edu/protocols/P21.html
  Added: Tue May 25 2004, Hits: 302, Reviews: 0  Write review   Cached
• Polyacrylamide Gel Electrophoresis of Oligonucleotides (John Roth, Division of Biological Sciences,University of California at Davis)
  http://rothlab.ucdavis.edu/protocols/E11.html
  Added: Sun Jun 06 2004, Hits: 2901, Reviews: 0  Write review   Cached
• Removal of 32P-ATP from Oligonucleoides (John Roth, Division of Biological Sciences,University of California at Davis)
  Column purification method
  http://rothlab.ucdavis.edu/protocols/P20.html
  Added: Tue May 25 2004, Hits: 258, Reviews: 0  Write review   Cached