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  • Agarose gel electrophoresis (basic method) (Matt Lewis, Department of Pathology, University of Liverpool )
    Agarose gel electrophoresis is the easiest and commonest way of separating and analyzing DNA. The purpose of the gel might be to look at the DNA, to quantify it or to isolate a particular band. The DNA is visualised in the gel by addition of ethidium bromide. This binds strongly to DNA by intercalating between the bases and is fluorescent meaning that it absorbs invisible UV light and transmits the energy as visible orange light.
    Besides a detailed protocol, also mentioned are sample preparation, molecular weight marker and loading buffer. Highly recommended.
    http://www.methodbook.net/dna/agarogel.html
    Added: Fri Sep 06 2002, Hits: 24139, Reviews: 1 Read reviews  Write review   Cached
  • Electrophoresis of DNA in Agarose Gels ( Dr. Bart Frank, Arthritis and Immunology Program, Oklahoma Medical Research Foundation)
    Detailed guide to DNA agarose gel electrophoresis from gel and sample preparation to photography
    http://omrf.ouhsc.edu/~frank/Agarose.html
    Added: Sat Jun 12 2004, Hits: 12335, Reviews: 0  Write review   Cached
  • Agarose Electrophoresis (Dr. Chen, Dept of Biochem. & Mol. Biology, Univ. College London)
    DNA electrophoresis, extraction and concentration determination.
    http://www.biochem.ucl.ac.uk/bsm/nmr/protocols/pro...
    Added: Tue May 14 2002, Hits: 9408, Reviews: 0  Write review   Cached
  • Agarose Gel (John Garland, The Biochemistry and Molecular Biology Department of The James H. Quillen College of Medicine in Johnson City, TN)
    How to prepare stock solutions and agarose solution
    http://qcom.etsu.edu/biochem/protocols/agarose.htm
    Added: Tue May 14 2002, Hits: 3830, Reviews: 0  Write review   Cached
  • Agarose Gel Electrophoresis (Molecular Biology Cyberlab)
    Very basic and illustrated guide
    http://www.life.uiuc.edu/molbio/geldigest/electro....
    Added: Tue May 14 2002, Hits: 8386, Reviews: 1 Read reviews  Write review   Cached
  • Agarose Gel Electrophoresis (Julie B. Wolf, University of Maryland, Baltimore County)
    Provides method in gel preparation, recipes of electrophoresis buffer and loading buffer...
    http://userpages.umbc.edu/~jwolf/m6.htm#agarose
    Added: Tue May 14 2002, Hits: 7072, Reviews: 0  Write review   Cached
  • Agarose Gel Electrophoresis (Frank Bottone, Jr.)
    http://www.unc.edu/~fbottone/protocol/electro.html
    Added: Tue May 14 2002, Hits: 4243, Reviews: 0  Write review   Cached
  • Agarose Gel Electrophoresis (Roe, OU)
    http://www.genome.ou.edu/protocol_book/protocol_pa...
    Added: Tue May 14 2002, Hits: 4869, Reviews: 0  Write review   Cached
  • Agarose Gel Electrophoresis (The Minion Lab, College of Veterinary Medicine at Iowa State University)
    Agarose gel electrophoresis is employed to check the progression of a restriction enzyme digestion, to quickly determine the yield and purity of a DNA isolation or PCR reaction, and to size fractionate DNA molecules. This protocol teaches you how to make a agarose gel.
    http://mycoplasmas.vm.iastate.edu/lab_site/methods...
    Added: Tue Nov 16 2004, Hits: 4119, Reviews: 0  Write review   Cached
  • Agarose Gel Electrophoresis of DNA (Dr. Chen, Dept of Biochem. & Mol. Biology, Univ. College London)
    Detailed protocol for gel preparation, electrophoresis and DNA extraction from gel.
    http://www.biochem.ucl.ac.uk/bsm/nmr/protocols/pro...
    Added: Tue May 14 2002, Hits: 6792, Reviews: 0  Write review   Cached
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